RRC ID 35383
Author Patil S, Valdramidis VP, Karatzas KA, Cullen PJ, Bourke P.
Title Assessing the microbial oxidative stress mechanism of ozone treatment through the responses of Escherichia coli mutants.
Journal J. Appl. Microbiol.
Abstract AIMS:To investigate the effect of the oxidative stress of ozone on the microbial inactivation, cell membrane integrity and permeability and morphology changes of Escherichia coli.
METHODS AND RESULTS:Escherichia coli BW 25113 and its isogenic mutants in soxR, soxS, oxyR, rpoS and dnaK genes were treated with ozone at a concentration of 6 μg ml⁻¹ for a period up to 240 s. A significant effect of ozone exposure on microbial inactivation was observed. After ozonation, minor effects on the cell membrane integrity and permeability were observed, while scanning electron microscopy analysis showed slightly altered cell surface structure.
CONCLUSIONS:The results of this study suggest that cell lysis was not the major mechanism of microbial inactivation. The deletion of oxidative stress-related genes resulted in increased susceptibility of E. coli cells to ozone treatment, implying that they play an important role for protection against the radicals produced by ozone. However, DnaK that has previously been shown to protect against oxidative stress did not protect against ozone treatment in this study. Furthermore, RpoS was important for the survival against ozone.
SIGNIFICANCE AND IMPACT OF THE STUDY:This study provides important information about the role of oxidative stress in the responses of E. coli during ozonation.
Volume 111(1)
Pages 136-44
Published 2011-7
DOI 10.1111/j.1365-2672.2011.05021.x
PMID 21457413
MeSH Cell Membrane / drug effects Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / ultrastructure Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Kinetics Microbial Viability / drug effects* Mutation Oxidative Stress Ozone / pharmacology*
IF 2.683
Times Cited 13
WOS Category BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY
Resource
Prokaryotes E. coli JW4024-KC JW4023-KC JW3933-KC JW5437-KC JW0013-KC ME9062(BW25113)