RRC ID 35386
Author Sobue R, Kurono N, Etchuya R, Maeda S.
Title Identification of a novel DNA element that promotes cell-to-cell transformation in Escherichia coli.
Journal FEBS Lett
Abstract Recently, we discovered a novel phenomenon, "cell-to-cell transformation" by which non-conjugative plasmids are transmitted horizontally in co-cultures of Escherichia coli F(-) strains. In this study, we aimed to identify the DNA element responsible for the high cell-to-cell transformability of pHSG299. By transplanting pHSG299 DNA fragments into pHSG399, a plasmid showing low transformability, we discovered that a specific 88 bp fragment of pHSG299 significantly promoted pHSG399 transformability. Although several short motif-like repetitive sequences (6-10 bp) were present in the 88 bp sequence, no known DNA motifs were recognized, suggesting that this 88 bp sequence (cell-to-cell transformation promoting sequence, CTPS; Accession number: AB634455) is a novel DNA element.
Volume 585(14)
Pages 2223-8
Published 2011-7-21
DOI 10.1016/j.febslet.2011.05.040
PII S0014-5793(11)00406-6
PMID 21621537
MeSH Base Sequence DNA Transposable Elements / genetics* DNA, Bacterial / genetics* Escherichia coli / genetics* Molecular Sequence Data Plasmids / genetics Plasmids / metabolism Transformation, Genetic*
IF 2.675
Times Cited 8
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Prokaryotes E. coli ME9033(DH5) ME8788(CAG18439) pHSG299 pHSG399 pUC19