RRC ID 35397
著者 Mulrooney SB, Howard MJ, Hausinger RP.
タイトル The Escherichia coli alkylation response protein AidB is a redox partner of flavodoxin and binds RNA and acyl carrier protein.
ジャーナル Arch Biochem Biophys
Abstract Microorganisms are exposed to a wide variety of exogenous and endogenous chemical agents that alkylate DNA. Escherichia coli cells exhibit an adaptive response that recognizes and repairs alkylated DNA lesions using Ada, AlkA, and AlkB enzymes. Another alkylation response protein, the DNA-binding flavoprotein AidB, was proposed to repair DNA or protect it from chemical alkylating agents, but direct evidence for its role is lacking. Here, AidB was shown to form tight complexes with both flavodoxin and acyl carrier protein. In addition, electron transfer between 1-electron and 2-electron reduced flavodoxin to oxidized AidB was observed, although with very small rate constants. AidB was found to bind to RNA, raising the prospect that the protein may have a role in protection of RNA from chemical alkylation. Finally, the reagent N-methyl-N'-nitro-N-nitrosoguanidine was eliminated as a direct substrate of the enzyme.
巻・号 513(2)
ページ 81-6
公開日 2011-9-15
DOI 10.1016/j.abb.2011.07.004
PII S0003-9861(11)00256-6
PMID 21782785
PMC PMC3166795
MeSH Acyl Carrier Protein / metabolism Alkylation Base Sequence DNA Repair DNA Repair Enzymes / chemistry DNA Repair Enzymes / genetics DNA Repair Enzymes / metabolism DNA, Bacterial / genetics DNA, Bacterial / metabolism Electron Transport Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / chemistry* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Flavodoxin / metabolism Methylnitronitrosoguanidine / metabolism Multiprotein Complexes / chemistry Multiprotein Complexes / metabolism Oxidation-Reduction Protein Binding RNA, Bacterial / chemistry RNA, Bacterial / metabolism Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Substrate Specificity
IF 3.391
引用数 2
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JW0671(ASKA) JW1080(ASKA)