論文 - 詳細
| RRC ID | 35413 |
|---|---|
| 著者 | Ollivierre JN, Sikora JL, Beuning PJ. |
| タイトル | The dimeric SOS mutagenesis protein UmuD is active as a monomer. |
| ジャーナル | J Biol Chem |
| Abstract |
The homodimeric umuD gene products play key roles in regulating the cellular response to DNA damage in Escherichia coli. UmuD(2) is composed of 139-amino acid subunits and is up-regulated as part of the SOS response. Subsequently, damage-induced RecA·ssDNA nucleoprotein filaments mediate the slow self-cleavage of the N-terminal 24-amino acid arms yielding UmuD'(2). UmuD(2) and UmuD'(2) make a number of distinct protein-protein contacts that both prevent and facilitate mutagenic translesion synthesis. Wild-type UmuD(2) and UmuD'(2) form exceptionally tight dimers in solution; however, we show that the single amino acid change N41D generates stable, active UmuD and UmuD' monomers that functionally mimic the dimeric wild-type proteins. The UmuD N41D monomer is proficient for cleavage and interacts physically with DNA polymerase IV (DinB) and the β clamp. Furthermore, the N41D variants facilitate UV-induced mutagenesis and promote overall cell viability. Taken together, these observations show that a monomeric form of UmuD retains substantial function in vivo and in vitro. |
| 巻・号 | 286(5) |
| ページ | 3607-17 |
| 公開日 | 2011-2-4 |
| DOI | 10.1074/jbc.M110.167254 |
| PII | S0021-9258(20)54065-9 |
| PMID | 21118802 |
| PMC | PMC3030365 |
| MeSH | Amino Acid Substitution Cell Survival DNA-Directed DNA Polymerase / genetics DNA-Directed DNA Polymerase / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Mutagenesis* / radiation effects Mutant Proteins / chemistry Mutant Proteins / metabolism Protein Multimerization* Protein Stability SOS Response, Genetics |
| IF | 4.238 |
| 引用数 | 11 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW2860-KC? |