論文 - 詳細
| RRC ID | 35433 |
|---|---|
| 著者 | Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T. |
| タイトル | Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC. |
| ジャーナル | J Mol Biol |
| Abstract |
The β-barrel assembly machinery (BAM) complex drives the assembly of β-barrel proteins into the outer membrane of gram-negative bacteria. It is composed of five subunits: BamA, BamB, BamC, BamD, and BamE. We find that the BAM complex isolated from the outer membrane of Escherichia coli consists of a core complex of BamA:B:C:D:E and, in addition, a BamA:B module and a BamC:D module. In the absence of BamC, these modules are destabilized, resulting in increased protease susceptibility of BamD and BamB. While the N-terminus of BamC carries a highly conserved region crucial for stable interaction with BamD, immunofluorescence, immunoprecipitation, and protease-sensitivity assays show that the C-terminal domain of BamC, composed of two helix-grip motifs, is exposed on the surface of E. coli. This unexpected topology of a bacterial lipoprotein is reminiscent of the analogous protein subunits from the mitochondrial β-barrel insertion machinery, the SAM complex. The modular arrangement and topological features provide new insight into the architecture of the BAM complex, towards a better understanding of the mechanism driving β-barrel membrane protein assembly. |
| 巻・号 | 422(4) |
| ページ | 545-55 |
| 公開日 | 2012-9-28 |
| DOI | 10.1016/j.jmb.2012.05.035 |
| PII | S0022-2836(12)00433-0 |
| PMID | 22683355 |
| PMC | PMC3433275 |
| MeSH | Amino Acid Sequence Bacterial Outer Membrane Proteins / metabolism* Escherichia coli / metabolism Escherichia coli Proteins / metabolism* Lipid-Linked Proteins / metabolism* Lipoproteins / metabolism* Models, Molecular Molecular Sequence Data Protein Structure, Secondary |
| IF | 4.76 |
| 引用数 | 43 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW2462-KC |