RRC ID 35439
著者 Sun Y, Fukamachi T, Saito H, Kobayashi H.
タイトル Respiration and the F₁Fo-ATPase enhance survival under acidic conditions in Escherichia coli.
ジャーナル PLoS One
Abstract Besides amino acid decarboxylation, the ADP biosynthetic pathway was reported to enhance survival under extremely acidic conditions in Escherichia coli (Sun et al., J. Bacteriol. 193∶ 3072-3077, 2011). E. coli has two pathways for ATP synthesis from ADP: glycolysis and oxidative phosphorylation. We found in this study that the deletion of the F(1)Fo-ATPase, which catalyzes the synthesis of ATP from ADP and inorganic phosphate using the electro-chemical gradient of protons generated by respiration in E. coli, decreased the survival at pH 2.5. A mutant deficient in hemA encoding the glutamyl tRNA reductase, which synthesizes glutamate 1-semialdehyde also showed the decreased survival of E. coli at pH 2.5. Glutamate 1-semialdehyde is a precursor of heme synthesis that is an essential component of the respiratory chain. The ATP content decreased rapidly at pH 2.5 in these mutants as compared with that of their parent strain. The internal pH was lowered by the deletion of these genes at pH 2.5. These results suggest that respiration and the F(1)Fo-ATPase are still working at pH 2.5 to enhance the survival under such extremely acidic conditions.
巻・号 7(12)
ページ e52577
公開日 2012-1-1
DOI 10.1371/journal.pone.0052577
PII PONE-D-12-25743
PMID 23300708
PMC PMC3534200
MeSH Adenosine Triphosphate / metabolism Escherichia coli / enzymology Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli / physiology Gene Expression Regulation, Bacterial Heme / biosynthesis Hydrogen-Ion Concentration Intracellular Space / chemistry Microbial Viability* Mutation Oxidative Phosphorylation Proton-Translocating ATPases / metabolism*
IF 2.74
引用数 0
WOS 分野 MICROBIOLOGY
リソース情報
原核生物(大腸菌) JW3710-KC JW3715-KC