RRC ID 35452
Author Ojima Y, Hakamada K, Nishinoue Y, Nguyen MH, Miyake J, Taya M.
Title Motility behavior of rpoS-deficient Escherichia coli analyzed by individual cell tracking.
Journal J Biosci Bioeng
Abstract Motility is one of the most extensively studied cellular events conducted by bacteria, including Escherichia coli. A motility agar plate assay showed that deletion of the rpoS gene enhanced the apparent motility of the E. coli BW25113 strain, which inherently had negligible motility compared to wild-type E. coli strains, such as MG1655, with no effect on cell growth. This enhancement of motility was accompanied by drastic up-regulation of genes involved in the formation and rotation of flagella. Furthermore, an individual cell motility assay showed that the population of ΔrpoS cells had bimodal motility character, and that a minority of this population exhibited a much higher motility rate. These results support a view that a minority population contributes to increasing in apparent motility of the whole population of ΔrpoS cells.
Volume 114(6)
Pages 652-6
Published 2012-12-1
DOI 10.1016/j.jbiosc.2012.06.014
PII S1389-1723(12)00281-2
PMID 22846441
MeSH Bacterial Proteins / genetics Biological Assay Cell Tracking Escherichia coli / cytology* Escherichia coli / genetics Escherichia coli / growth & development Escherichia coli / physiology* Escherichia coli Proteins / genetics Flagella / genetics Flagella / physiology Gene Deletion Gene Expression Regulation, Bacterial Movement* Sigma Factor / deficiency* Sigma Factor / genetics Up-Regulation
IF 2.366
Times Cited 4
WOS Category FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Resource
Prokaryotes E. coli NA