| RRC ID |
35479
|
| Author |
Kimura S, Ikeuchi Y, Kitahara K, Sakaguchi Y, Suzuki T, Suzuki T.
|
| Title |
Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity.
|
| Journal |
Nucleic Acids Res
|
| Abstract |
Modifications of rRNAs are clustered in functional regions of the ribosome. In Helix 74 of Escherichia coli 23S rRNA, guanosines at positions 2069 and 2445 are modified to 7-methylguanosine(m(7)G) and N(2)-methylguanosine(m(2)G), respectively. We searched for the gene responsible for m(7)G2069 formation, and identified rlmL, which encodes the methyltransferase for m(2)G2445, as responsible for the biogenesis of m(7)G2069. In vitro methylation of rRNA revealed that rlmL encodes a fused methyltransferase responsible for forming both m(7)G2069 and m(2)G2445. We renamed the gene rlmKL. The N-terminal RlmL activity for m(2)G2445 formation was significantly enhanced by the C-terminal RlmK. Moreover, RlmKL had an unwinding activity of Helix 74, facilitating cooperative methylations of m(7)G2069 and m(2)G2445 during biogenesis of 50S subunit. In fact, we observed that RlmKL was involved in the efficient assembly of 50S subunit in a mutant strain lacking an RNA helicase deaD.
|
| Volume |
40(9)
|
| Pages |
4071-85
|
| Published |
2012-5-1
|
| DOI |
10.1093/nar/gkr1287
|
| PII |
gkr1287
|
| PMID |
22210896
|
| PMC |
PMC3351187
|
| MeSH |
Escherichia coli / enzymology
Escherichia coli / genetics
Escherichia coli Proteins / chemistry
Escherichia coli Proteins / metabolism*
Guanosine / analogs & derivatives*
Guanosine / metabolism
Methylation
Methyltransferases / chemistry
Methyltransferases / metabolism*
Models, Molecular
Protein Structure, Tertiary
RNA Helicases / metabolism
RNA, Double-Stranded / chemistry
RNA, Double-Stranded / metabolism*
RNA, Ribosomal, 23S / chemistry
RNA, Ribosomal, 23S / metabolism*
Ribosome Subunits, Large, Bacterial / metabolism
|
| IF |
11.502
|
| Times Cited |
21
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Prokaryotes E. coli |
JW0931-KC
OCL/R-series
ME4805(OCR55) |
