RRC ID 3551
著者 Tanizawa Y, Kuhara A, Inada H, Kodama E, Mizuno T, Mori I.
タイトル Inositol monophosphatase regulates localization of synaptic components and behavior in the mature nervous system of C. elegans.
ジャーナル Genes Dev
Abstract Although recent studies have provided significant molecular insights into the establishment of neuronal polarity in vitro, evidence is lacking on the corresponding phenomena in vivo, including correct localization of synaptic components and the importance of this process for function of the nervous system as a whole. RIA interneurons act as a pivotal component of the neural circuit for thermotaxis behavior in the nematode Caenorhabditis elegans and provide a suitable model to investigate these issues, having a neurite clearly divided into pre- and post-synaptic regions. In a screen for thermotaxis mutants, we identified the gene ttx-7, which encodes myo-inositol monophosphatase (IMPase), an inositol-producing enzyme regarded as a bipolar disorder-relevant molecule for its lithium sensitivity. Here we show that mutations in ttx-7 cause defects in thermotaxis behavior and localization of synaptic proteins in RIA neurons in vivo. Both behavioral and localization defects in ttx-7 mutants were rescued by expression of IMPase in adults and by inositol application, and the same defects were mimicked by lithium treatment in wild-type animals. These results suggest that IMPase is required in central interneurons of the mature nervous system for correct localization of synaptic components and thus for normal behavior.
巻・号 20(23)
ページ 3296-310
公開日 2006-12-1
DOI 10.1101/gad.1497806
PII 20/23/3296
PMID 17158747
PMC PMC1686606
MeSH Amino Acid Sequence Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / growth & development Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Movement Conserved Sequence Interneurons / enzymology Interneurons / physiology* Molecular Sequence Data Nervous System Physiological Phenomena* Neurons / enzymology Neurons / physiology Phosphoric Monoester Hydrolases / chemistry Phosphoric Monoester Hydrolases / genetics Phosphoric Monoester Hydrolases / metabolism* Phylogeny Sequence Alignment Sequence Homology, Amino Acid Synapses / physiology*
IF 9.527
引用数 53
WOS 分野 GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
線虫 tm2223