論文 - 詳細
| RRC ID | 35516 |
|---|---|
| 著者 | Yamamoto S, Suzuki K. |
| タイトル | Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains. |
| ジャーナル | J Microbiol Methods |
| Abstract |
For curing stable Ti plasmids from Agrobacterium strains, we developed a new Ti-eviction plasmid. The plasmid contains two antitoxin genes, which originated from two toxin-antitoxin systems in Ti plasmids. The plasmid facilitated production of Ti-less cells in seven tested strains, and was removed easily by simple counter-selection from the cells. |
| 巻・号 | 89(1) |
| ページ | 53-6 |
| 公開日 | 2012-4-1 |
| DOI | 10.1016/j.mimet.2012.01.019 |
| PII | S0167-7012(12)00037-1 |
| PMID | 22326751 |
| MeSH | Agrobacterium / genetics* Bacterial Toxins Genomic Instability Microbial Viability Plant Tumor-Inducing Plasmids* Selection, Genetic Sequence Deletion |
| IF | 1.707 |
| 引用数 | 2 |
| WOS 分野 | MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS |
| リソース情報 | |
| 原核生物(大腸菌) | NA |