Reference - Detail
| RRC ID | 35530 |
|---|---|
| Author | Rathnasingh C, Raj SM, Lee Y, Catherine C, Ashok S, Park S. |
| Title | Production of 3-hydroxypropionic acid via malonyl-CoA pathway using recombinant Escherichia coli strains. |
| Journal | J Biotechnol |
| Abstract |
Malonyl-CoA is an intermediary compound that is produced during fatty acid metabolism. Our study aimed to produce the commercially important platform chemical 3-hydroxypropionic acid (3-HP) from its immediate precursor malonyl-CoA by recombinant Escherichia coli strains heterologously expressing the mcr gene of Chloroflexus aurantiacus DSM 635, encoding an NADPH-dependent malonyl-CoA reductase (MCR). The recombinant E. coli overexpressing mcr under the T5 promoter showed MCR activity of 0.015 U mg⁻¹ protein in crude cell extract and produced 0.71 mmol/L of 3-HP in 24h in shake flask cultivation under aerobic conditions with glucose as the sole source of carbon. When acetyl-CoA carboxylase and biotinilase, encoded by the genes accADBCb (ACC) of E. coli K-12 were overexpressed along with MCR, the final 3-HP titer improved by 2-fold, which is 1.6 mM. Additional expression of the gene pntAB, encoding nicotinamide nucleotide transhydrogenase that converts NADH to NADPH, increased 3-HP production to 2.14 mM. The strain was further developed by deleting the sucAB gene, encoding α-ketoglutarate dehydrogenase complex in tricarboxylic acid (TCA) cycle, or blocking lactate and acetate production pathways, and evaluated for the production of 3-HP. We report on the feasibility of producing 3-HP from glucose through the malonyl-CoA pathway. |
| Volume | 157(4) |
| Pages | 633-40 |
| Published | 2012-2-20 |
| DOI | 10.1016/j.jbiotec.2011.06.008 |
| PII | S0168-1656(11)00307-5 |
| PMID | 21723339 |
| MeSH | Acetyl-CoA Carboxylase / metabolism Aerobiosis Anaerobiosis Escherichia coli / cytology Escherichia coli / enzymology Escherichia coli / genetics* Escherichia coli / growth & development Gene Deletion Glucose / metabolism Lactic Acid / analogs & derivatives* Lactic Acid / biosynthesis Malonyl Coenzyme A / metabolism* Metabolic Networks and Pathways* NADP Transhydrogenases / metabolism Oxidoreductases / metabolism Plasmids / genetics Recombination, Genetic / genetics* Temperature Time Factors |
| IF | 3.503 |
| Times Cited | 99 |
| WOS Category | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 44 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | |