論文 - 詳細
| RRC ID | 35541 |
|---|---|
| 著者 | Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Lee WK, Chung WJ. |
| タイトル | Biosynthesis of ethylene glycol in Escherichia coli. |
| ジャーナル | Appl Microbiol Biotechnol |
| Abstract |
Ethylene glycol (EG) is an important platform chemical with steadily expanding global demand. Its commercial production is currently limited to fossil resources; no biosynthesis route has been delineated. Herein, a biosynthesis route for EG production from D-xylose is reported. This route consists of four steps: D-xylose → D-xylonate → 2-dehydro-3-deoxy-D-pentonate → glycoaldehyde → EG. Respective enzymes, D-xylose dehydrogenase, D-xylonate dehydratase, 2-dehydro-3-deoxy-D-pentonate aldolase, and glycoaldehyde reductase, were assembled. The route was implemented in a metabolically engineered Escherichia coli, in which the D-xylose → D-xylulose reaction was prevented by disrupting the D-xylose isomerase gene. The most efficient construct produced 11.7 g L(-1) of EG from 40.0 g L(-1) of D-xylose. Glycolate is a carbon-competing by-product during EG production in E. coli; blockage of glycoaldehyde → glycolate reaction was also performed by disrupting the gene encoding aldehyde dehydrogenase, but from this approach, EG productivity was not improved but rather led to D-xylonate accumulation. To channel more carbon flux towards EG than the glycolate pathway, further systematic metabolic engineering and fermentation optimization studies are still required to improve EG productivity. |
| 巻・号 | 97(8) |
| ページ | 3409-17 |
| 公開日 | 2013-4-1 |
| DOI | 10.1007/s00253-012-4618-7 |
| PMID | 23233208 |
| MeSH | Biosynthetic Pathways / genetics* Escherichia coli / genetics* Escherichia coli / metabolism* Ethylene Glycol / metabolism* Metabolic Engineering / methods Xylose / metabolism |
| IF | 3.53 |
| 引用数 | 52 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 25 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW1412-KC |