RRC ID 35542
Author Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Malihan LB, Lee WK, Park SJ, Chung WJ.
Title Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate.
Journal Bioprocess Biosyst Eng
Abstract D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L(-1) D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L(-1) D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L(-1) and yield of 62.0 %. Batch fermentation in 20 g L(-1) D-galactose of E. coli ∆galK∆dgoK mutant expressing the gld resulted in 17.6 g L(-1) of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate.
Volume 37(3)
Pages 383-91
Published 2014-3-1
DOI 10.1007/s00449-013-1003-6
PMID 23820824
MeSH Base Sequence Cloning, Molecular Culture Media DNA Primers Escherichia coli / genetics Escherichia coli / metabolism* Galactose Dehydrogenases / genetics Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Molecular Structure Pseudomonas syringae / enzymology Sugar Acids / chemistry Sugar Acids / metabolism*
IF 2.419
Times Cited 5
Prokaryotes E. coli ME9062(BW25113) JW0740-KC(galK)