論文 - 詳細
| RRC ID | 35542 |
|---|---|
| 著者 | Liu H, Ramos KR, Valdehuesa KN, Nisola GM, Malihan LB, Lee WK, Park SJ, Chung WJ. |
| タイトル | Metabolic engineering of Escherichia coli for biosynthesis of D-galactonate. |
| ジャーナル | Bioprocess Biosyst Eng |
| Abstract |
D-galactose is an attractive substrate for bioconversion. Herein, Escherichia coli was metabolically engineered to convert D-galactose into D-galactonate, a valuable compound in the polymer and cosmetic industries. D-galactonate productions by engineered E. coli strains were observed in shake flask cultivations containing 2 g L(-1) D-galactose. Engineered E. coli expressing gld coding for galactose dehydrogenase from Pseudomonas syringae was able to produce 0.17 g L(-1) D-galactonate. Inherent metabolic pathways for assimilating both D-galactose and D-galactonate were blocked to enhance the production of D-galactonate. This approach finally led to a 7.3-fold increase with D-galactonate concentration of 1.24 g L(-1) and yield of 62.0 %. Batch fermentation in 20 g L(-1) D-galactose of E. coli ∆galK∆dgoK mutant expressing the gld resulted in 17.6 g L(-1) of D-galactonate accumulation and highest yield of 88.1 %. Metabolic engineering strategy developed in this study could be useful for industrial production of D-galactonate. |
| 巻・号 | 37(3) |
| ページ | 383-91 |
| 公開日 | 2014-3-1 |
| DOI | 10.1007/s00449-013-1003-6 |
| PMID | 23820824 |
| MeSH | Base Sequence Cloning, Molecular Culture Media DNA Primers Escherichia coli / genetics Escherichia coli / metabolism* Galactose Dehydrogenases / genetics Hydrogen-Ion Concentration Magnetic Resonance Spectroscopy Molecular Structure Pseudomonas syringae / enzymology Sugar Acids / chemistry Sugar Acids / metabolism* |
| IF | 2.419 |
| 引用数 | 5 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY ENGINEERING, CHEMICAL |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062(BW25113) JW0740-KC(galK) |