RRC ID 35552
Author Kubo I, Hosoda K, Suzuki S, Yamamoto K, Kihara K, Mori K, Yomo T.
Title Construction of bacteria-eukaryote synthetic mutualism.
Journal Biosystems
Abstract Mutualism is ubiquitous in nature but is known to be intrinsically vulnerable with regard to both population dynamics and evolution. Synthetic ecology has indicated that it is feasible for organisms to establish novel mutualism merely through encountering each other by showing that it is feasible to construct synthetic mutualism between organisms. However, bacteria-eukaryote mutualism, which is ecologically important, has not yet been constructed. In this study, we synthetically constructed mutualism between a bacterium and a eukaryote by using two model organisms. We mixed a bacterium, Escherichia coli (a genetically engineered glutamine auxotroph), and an amoeba, Dictyostelium discoideum, in 14 sets of conditions in which each species could not grow in monoculture but potentially could grow in coculture. Under a single condition in which the bacterium and amoeba mutually compensated for the lack of required nutrients (lipoic acid and glutamine, respectively), both species grew continuously through several subcultures, essentially establishing mutualism. Our results shed light on the establishment of bacteria-eukaryote mutualism and indicate that a bacterium and eukaryote pair in nature also has a non-negligible possibility of establishing novel mutualism if the organisms are potentially mutualistic.
Volume 113(2)
Pages 66-71
Published 2013-8-1
DOI 10.1016/j.biosystems.2013.05.006
PII S0303-2647(13)00131-7
PMID 23711432
MeSH Dictyostelium / growth & development* Escherichia coli / growth & development* Population Dynamics Symbiosis / physiology* Synthetic Biology / methods*
IF 1.808
Times Cited 11
Prokaryotes E. coli ME8569(DH1)