Reference - Detail
| RRC ID | 3556 |
|---|---|
| Author | Yamauchi S, Yamanaka K, Ogura T. |
| Title | Comparative analysis of expression of two p97 homologues in Caenorhabditis elegans. |
| Journal | Biochem Biophys Res Commun |
| Abstract |
Caenorhabditis elegans possesses two p97/VCP/Cdc48p homologues, named CDC-48.1 (C06A1.1) and CDC-48.2 (C41C4.8), although their expression regulation and functional diversity have not yet been studied. We therefore investigated spatial and temporal expression patterns of two p97 homologues in this study. RT-PCR and Western blot analysis showed that the amount of cdc-48.1 was about twofold of that of cdc-48.2 in adults and that two p97 homologues were induced by ER stress. The amount of cdc-48.1 mRNA did not increase in the cdc-48.2 deletion mutant and vice versa. In situ hybridization showed that two p97 homologues are mainly expressed in germ cells. In vivo expression analysis by using GFP translational fusion constructs revealed that CDC-48.1::GFP was expressed from embryos through to adult worms, while CDC-48.2::GFP was expressed mainly in embryos. These results suggest that the expression of two p97 homologues of C. elegans is differently regulated and independent of each other. |
| Volume | 345(2) |
| Pages | 746-53 |
| Published | 2006-6-30 |
| DOI | 10.1016/j.bbrc.2006.04.160 |
| PII | S0006-291X(06)00971-5 |
| PMID | 16701565 |
| MeSH | Adenosine Triphosphatases Animals Base Sequence Blotting, Western Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology Cell Cycle Proteins / analysis* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Endoplasmic Reticulum / metabolism Endoplasmic Reticulum / pathology Gene Expression Regulation* In Situ Hybridization Mutation RNA, Messenger / genetics RNA, Messenger / metabolism Reverse Transcriptase Polymerase Chain Reaction Valosin Containing Protein |
| IF | 2.985 |
| Times Cited | 17 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| C.elegans | tm544 tm659 |