RRC ID 35578
Author Arora S, Bhamidimarri SP, Weber MH, Varshney U.
Title Role of the ribosomal P-site elements of m²G966, m⁵C967, and the S9 C-terminal tail in maintenance of the reading frame during translational elongation in Escherichia coli.
Journal J Bacteriol
Abstract The ribosomal P-site hosts the peptidyl-tRNAs during translation elongation. Which P-site elements support these tRNA species to maintain codon-anticodon interactions has remained unclear. We investigated the effects of P-site features of methylations of G966, C967, and the conserved C-terminal tail sequence of Ser, Lys, and Arg (SKR) of the S9 ribosomal protein in maintenance of the translational reading frame of an mRNA. We generated Escherichia coli strains deleted for the SKR sequence in S9 ribosomal protein, RsmB (which methylates C967), and RsmD (which methylates G966) and used them to translate LacZ from its +1 and -1 out-of-frame constructs. We show that the S9 SKR tail prevents both the +1 and -1 frameshifts and plays a general role in holding the P-site tRNA/peptidyl-tRNA in place. In contrast, the G966 and C967 methylations did not make a direct contribution to the maintenance of the translational frame of an mRNA. However, deletion of rsmB in the S9Δ3 background caused significantly increased -1 frameshifting at 37°C. Interestingly, the effects of the deficiency of C967 methylation were annulled when the E. coli strain was grown at 30°C, supporting its context-dependent role.
Volume 195(16)
Pages 3524-30
Published 2013-8-1
DOI 10.1128/JB.00455-13
PII JB.00455-13
PMID 23729652
PMC PMC3754560
MeSH Amino Acid Sequence Codon Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Frameshift Mutation Gene Expression Regulation, Bacterial / physiology* Methylation Models, Molecular Peptide Chain Elongation, Translational / physiology* Protein Conformation Ribosomes Thermus thermophilus / genetics Thermus thermophilus / metabolism
IF 3.006
Times Cited 7
Prokaryotes E. coli ME9062(BW25113)