RRC ID 35583
著者 Mehta P, Jovanovic G, Lenn T, Bruckbauer A, Engl C, Ying L, Buck M.
タイトル Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells.
ジャーナル Nat Commun
Abstract Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF-PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF hexamer-DNA complex and regulation of the psp promoters.
巻・号 4
ページ 1997
公開日 2013-1-1
DOI 10.1038/ncomms2997
PII ncomms2997
PMID 23764692
PMC PMC3709507
MeSH Bacterial Proteins / metabolism DNA-Binding Proteins / metabolism* Enhancer Elements, Genetic / genetics* Escherichia coli / cytology* Escherichia coli / genetics* Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Heat-Shock Proteins / metabolism Microbial Viability* Promoter Regions, Genetic Protein Binding RNA Polymerase Sigma 54 / metabolism Stress, Physiological Trans-Activators / metabolism* Transcription, Genetic
IF 12.121
引用数 20
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) JW3169? ME9062(BW25113)?