RRC ID |
35583
|
著者 |
Mehta P, Jovanovic G, Lenn T, Bruckbauer A, Engl C, Ying L, Buck M.
|
タイトル |
Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells.
|
ジャーナル |
Nat Commun
|
Abstract |
Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF-PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF hexamer-DNA complex and regulation of the psp promoters.
|
巻・号 |
4
|
ページ |
1997
|
公開日 |
2013-1-1
|
DOI |
10.1038/ncomms2997
|
PII |
ncomms2997
|
PMID |
23764692
|
PMC |
PMC3709507
|
MeSH |
Bacterial Proteins / metabolism
DNA-Binding Proteins / metabolism*
Enhancer Elements, Genetic / genetics*
Escherichia coli / cytology*
Escherichia coli / genetics*
Escherichia coli Proteins / metabolism*
Gene Expression Regulation, Bacterial
Heat-Shock Proteins / metabolism
Microbial Viability*
Promoter Regions, Genetic
Protein Binding
RNA Polymerase Sigma 54 / metabolism
Stress, Physiological
Trans-Activators / metabolism*
Transcription, Genetic
|
IF |
12.121
|
引用数 |
20
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
原核生物(大腸菌) |
JW3169?
ME9062(BW25113)? |