論文 - 詳細
| RRC ID | 35583 |
|---|---|
| 著者 | Mehta P, Jovanovic G, Lenn T, Bruckbauer A, Engl C, Ying L, Buck M. |
| タイトル | Dynamics and stoichiometry of a regulated enhancer-binding protein in live Escherichia coli cells. |
| ジャーナル | Nat Commun |
| Abstract |
Bacterial enhancer-dependent transcription systems support major adaptive responses and offer a singular paradigm in gene control analogous to complex eukaryotic systems. Here we report new mechanistic insights into the control of one-membrane stress-responsive bacterial enhancer-dependent system. Using millisecond single-molecule fluorescence microscopy of live cells we determine the localizations, two-dimensional diffusion dynamics and stoichiometries of complexes of the bacterial enhancer-binding ATPase PspF during its action at promoters as regulated by inner membrane interacting negative controller PspA. We establish that a stable repressive PspF-PspA complex is located in the nucleoid, transiently communicating with the inner membrane via PspA. The PspF as a hexamer stably binds only one of the two psp promoters at a time, suggesting that psp promoters will fire asynchronously and cooperative interactions of PspF with the basal transcription complex influence dynamics of the PspF hexamer-DNA complex and regulation of the psp promoters. |
| 巻・号 | 4 |
| ページ | 1997 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/ncomms2997 |
| PII | ncomms2997 |
| PMID | 23764692 |
| PMC | PMC3709507 |
| MeSH | Bacterial Proteins / metabolism DNA-Binding Proteins / metabolism* Enhancer Elements, Genetic / genetics* Escherichia coli / cytology* Escherichia coli / genetics* Escherichia coli Proteins / metabolism* Gene Expression Regulation, Bacterial Heat-Shock Proteins / metabolism Microbial Viability* Promoter Regions, Genetic Protein Binding RNA Polymerase Sigma 54 / metabolism Stress, Physiological Trans-Activators / metabolism* Transcription, Genetic |
| IF | 12.121 |
| 引用数 | 20 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | JW3169? ME9062(BW25113)? |