論文 - 詳細
| RRC ID | 35619 |
|---|---|
| 著者 | Shah AA, Wang C, Chung YR, Kim JY, Choi ES, Kim SW. |
| タイトル | Enhancement of geraniol resistance of Escherichia coli by MarA overexpression. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Improvement of a microorganism's tolerance against organic solvents is required for a microbial factory producing terpenoid based biofuels. The bacterial genes, marA, imp, cls and cti have been found to increase organic solvent tolerance. Thus, the tolerance against the following terpenoids (isopentenol, geraniol, myrcene, and farnesol) was studied with overexpression of marA, imp, cls and cti genes in Escherichia coli. The marA overexpression significantly enhanced the tolerance of E. coli against geraniol, whereas there was no tolerance improvement against the terpenoids by overexpression of cls and cti genes. The imp overexpression even yielded sensitive phenotype to the tested solvents. The colony forming efficiency of the marA overexpressing E. coli was increased by 10(4)-fold in plate overlay of geraniol compared to that of wild type E. coli and a two-fold decrease of intracellular geraniol accumulation was also observed in liquid culture of geraniol. Single knock-out mutations of marA, or one of the following genes (acrA, acrB and tolC) encoding AcrAB-TolC efflux pump made E. coli hypersensitive to geraniol. The geraniol tolerance conferred by marA overexpression was attributed to the AcrAB-TolC efflux pump that is activated by MarA. |
| 巻・号 | 115(3) |
| ページ | 253-8 |
| 公開日 | 2013-3-1 |
| DOI | 10.1016/j.jbiosc.2012.10.009 |
| PII | S1389-1723(12)00418-5 |
| PMID | 23168261 |
| MeSH | Acyclic Monoterpenes Bacterial Outer Membrane Proteins / genetics Bacterial Outer Membrane Proteins / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Drug Resistance, Bacterial Escherichia coli / drug effects* Escherichia coli / genetics Escherichia coli / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism* Genes, Bacterial Lipoproteins / metabolism Membrane Transport Proteins / metabolism Multidrug Resistance-Associated Proteins / metabolism Mutation Solvents / toxicity* Terpenes / metabolism Terpenes / toxicity* |
| IF | 2.366 |
| 引用数 | 18 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | NA |