| RRC ID |
35620
|
| Author |
Shah AA, Wang C, Yoon SH, Kim JY, Choi ES, Kim SW.
|
| Title |
RecA-mediated SOS response provides a geraniol tolerance in Escherichia coli.
|
| Journal |
J Biotechnol
|
| Abstract |
Geraniol is an important industrial material and a potential candidate of advanced biofuels. One challenge of microbial geraniol production is the toxicity to hosts. However, the poor understanding on geraniol tolerance mechanism is an obstacle for developing geraniol tolerant host. This study genome-widely screened a shot-gun DNA library of Escherichia coli and found that recA is able to confer geraniol tolerance in E. coli. The recA knockout mutant was found extremely sensitive to geraniol. Based on our data, it was deciphered that recA provided tolerance through SOS response network responding to DNA damage caused by geraniol. RecA-mediated SOS response activates the homologous recombinational repair by RecB and RecN for corrective DNA maintenance. This protection mechanism suggests an effective strategy to combat geraniol toxicity in E. coli.
|
| Volume |
167(4)
|
| Pages |
357-64
|
| Published |
2013-9-20
|
| DOI |
10.1016/j.jbiotec.2013.07.023
|
| PII |
S0168-1656(13)00329-5
|
| PMID |
23906844
|
| MeSH |
Acyclic Monoterpenes
Biofuels
DNA Repair*
DNA, Bacterial / genetics
DNA, Bacterial / metabolism
Drug Resistance, Bacterial
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli / metabolism*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism*
Gene Knockout Techniques
Gene Library
Genome, Bacterial
Microbial Viability / drug effects
Rec A Recombinases / genetics
Rec A Recombinases / metabolism*
Recombinational DNA Repair / genetics
SOS Response, Genetics*
Terpenes / toxicity*
|
| IF |
3.503
|
| Times Cited |
8
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Prokaryotes E. coli |
JW2669-KC
JW2788-KC
JW3784-KC
JW5416-KC
ME9062(BW25113)
pCP20 |
