RRC ID 35630
Author Pradenas GA, Díaz-Vásquez WA, Pérez-Donoso JM, Vásquez CC.
Title Monounsaturated fatty acids are substrates for aldehyde generation in tellurite-exposed Escherichia coli.
Journal Biomed Res Int
Abstract Reactive oxygen species (ROS) damage macromolecules and cellular components in nearly all kinds of cells and often generate toxic intracellular byproducts. In this work, aldehyde generation derived from the Escherichia coli membrane oxidation as well as membrane fatty acid profiles, protein oxidation, and bacterial resistance to oxidative stress elicitors was evaluated. Studies included wild-type cells as well as cells exhibiting a modulated monounsaturated fatty acid (MUFA) ratio. The hydroxyaldehyde 4-hydroxy 2-nonenal was found to be most likely produced by E. coli, whose levels are dependent upon exposure to oxidative stress elicitors. Aldehyde amounts and markers of oxidative damage decreased upon exposure to E. coli containing low MUFA ratios, which was paralleled by a concomitant increase in resistance to ROS-generating compounds. MUFAs ratio, lipid peroxidation, and aldehyde generation were found to be directly related; that is, the lower the MUFAs ratio, the lower the peroxide and aldehyde generation levels. These results provide additional evidence about MUFAs being targets for membrane lipid oxidation and their relevance in aldehyde generation.
Volume 2013
Pages 563756
Published 2013
DOI 10.1155/2013/563756
PMID 23991420
PMC PMC3749545
MeSH Aldehydes / metabolism* Escherichia coli / drug effects Escherichia coli / physiology* Fatty Acids, Monounsaturated / metabolism* Membrane Lipids / metabolism* Oxidation-Reduction / drug effects Oxidative Stress / drug effects Oxidative Stress / physiology* Reactive Oxygen Species / metabolism* Tellurium / pharmacology*
IF 2.583
Resource
Prokaryotes E. coli ?