論文 - 詳細
| RRC ID | 3567 |
|---|---|
| 著者 | Couwenbergs C, Labbé JC, Goulding M, Marty T, Bowerman B, Gotta M. |
| タイトル | Heterotrimeric G protein signaling functions with dynein to promote spindle positioning in C. elegans. |
| ジャーナル | J Cell Biol |
| Abstract |
Proper orientation and positioning of the mitotic spindle is essential for the correct segregation of fate determinants during asymmetric cell division. Although heterotrimeric G proteins and their regulators are essential for spindle positioning in many cell types, their mechanism of action remains unclear. In this study, we show that dyrb-1, which encodes a dynein light chain, provides a functional link between heterotrimeric G protein signaling and dynein activity during spindle positioning in Caenorhabditis elegans. Embryos depleted of dyrb-1 display phenotypes similar to a weak loss of function of dynein activity, indicating that DYRB-1 is a positive regulator of dynein. We find that the depletion of dyrb-1 enhances the spindle positioning defect of weak loss of function alleles of two regulators of G protein signaling, LIN-5 and GPR-1/2, and that DYRB-1 physically associates with these two proteins. These results indicate that dynein activity functions with regulators of G protein signaling to regulate common downstream effectors during spindle positioning in the early C. elegans embryo. |
| 巻・号 | 179(1) |
| ページ | 15-22 |
| 公開日 | 2007-10-8 |
| DOI | 10.1083/jcb.200707085 |
| PII | jcb.200707085 |
| PMID | 17908918 |
| PMC | PMC2064726 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans / ultrastructure Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / metabolism Caenorhabditis elegans Proteins / physiology* Dyneins / antagonists & inhibitors Dyneins / metabolism* Dyneins / physiology Embryo, Nonmammalian / metabolism Embryo, Nonmammalian / ultrastructure Green Fluorescent Proteins / analysis Heterotrimeric GTP-Binding Proteins / metabolism Heterotrimeric GTP-Binding Proteins / physiology* Models, Biological RNA Interference Recombinant Fusion Proteins / analysis Signal Transduction* Spindle Apparatus / metabolism* Spindle Apparatus / ultrastructure |
| IF | 8.811 |
| 引用数 | 87 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 線虫 | tm2645 |