RRC ID 35710
Author Ujino S, Nishitsuji H, Hishiki T, Sugiyama K, Takaku H, Shimotohno K.
Title Hepatitis C virus utilizes VLDLR as a novel entry pathway.
Journal Proc Natl Acad Sci U S A
Abstract Various host factors are involved in the cellular entry of hepatitis C virus (HCV). In addition to the factors previously reported, we discovered that the very-low-density lipoprotein receptor (VLDLR) mediates HCV entry independent of CD81. Culturing Huh7.5 cells under hypoxic conditions significantly increased HCV entry as a result of the expression of VLDLR, which was not expressed under normoxic conditions in this cell line. Ectopic VLDLR expression conferred susceptibility to HCV entry of CD81-deficient Huh7.5 cells. Additionally, VLDLR-mediated HCV entry was not affected by the knockdown of cellular factors known to act as HCV receptors or HCV entry factors. Because VLDLR is expressed in primary human hepatocytes, our results suggest that VLDLR functions in vivo as an HCV receptor independent of canonical CD81-mediated HCV entry.
Volume 113(1)
Pages 188-93
Published 2016-1-5
DOI 10.1073/pnas.1506524113
PII 1506524113
PMID 26699506
PMC PMC4711846
MeSH Anaerobiosis Animals Cell Line Hepacivirus / physiology* Hepatitis C / virology* Hepatocytes / virology* Humans Lipoproteins, VLDL / metabolism Mice Mice, Transgenic Occludin / genetics Receptors, LDL / genetics Receptors, LDL / physiology* Receptors, Virus / genetics Receptors, Virus / physiology* Tetraspanin 28 / genetics Tetraspanin 28 / physiology Virus Internalization*
IF 9.412
Times Cited 27
DNA material CSII-CMV-MCS (RDB04377) pRSV-Rev (RDB08121) pCAG-HIVgp (RDB04394) pCMV-VSV-G (RDB04392)
Human and Animal Cells Hepa 1-6(RCB1638)