RRC ID |
35752
|
Author |
Yamamoto N, Oshima M, Tanaka C, Ogawa M, Nakajima K, Ishida K, Moriyama K, Tsuji T.
|
Title |
Functional tooth restoration utilising split germs through re-regionalisation of the tooth-forming field.
|
Journal |
Sci Rep
|
Abstract |
The tooth is an ectodermal organ that arises from a tooth germ under the regulation of reciprocal epithelial-mesenchymal interactions. Tooth morphogenesis occurs in the tooth-forming field as a result of reaction-diffusion waves of specific gene expression patterns. Here, we developed a novel mechanical ligation method for splitting tooth germs to artificially regulate the molecules that control tooth morphology. The split tooth germs successfully developed into multiple correct teeth through the re-regionalisation of the tooth-forming field, which is regulated by reaction-diffusion waves in response to mechanical force. Furthermore, split teeth erupted into the oral cavity and restored physiological tooth function, including mastication, periodontal ligament function and responsiveness to noxious stimuli. Thus, this study presents a novel tooth regenerative technology based on split tooth germs and the re-regionalisation of the tooth-forming field by artificial mechanical force.
|
Volume |
5
|
Pages |
18393
|
Published |
2015-12-17
|
DOI |
10.1038/srep18393
|
PII |
srep18393
|
PMID |
26673152
|
PMC |
PMC4682098
|
MeSH |
Animals
Biomechanical Phenomena / genetics
Biomechanical Phenomena / physiology
Dental Restoration, Permanent / methods*
Dental Restoration, Temporary / methods*
Gene Expression / genetics
In Situ Hybridization
Mice, Inbred C57BL
Mice, Transgenic
Odontogenesis / genetics
Odontogenesis / physiology*
Periodontal Ligament / metabolism
Periodontal Ligament / physiology
Periodontal Ligament / surgery
Regeneration / genetics
Regeneration / physiology
Reverse Transcriptase Polymerase Chain Reaction
Tooth / metabolism
Tooth / physiology*
Tooth / surgery
Tooth Germ / metabolism
Tooth Germ / physiology*
Tooth Germ / surgery
|
IF |
3.998
|
Times Cited |
6
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
|
Resource |
Mice |
RBRC02707 |