RRC ID 35766
著者 Shiomi D, Toyoda A, Aizu T, Ejima F, Fujiyama A, Shini T, Kohara Y, Niki H.
タイトル Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells.
ジャーナル Mol Microbiol
Abstract RodZ interacts with MreB and both factors are required to maintain the rod shape of Escherichia coli. The assembly of MreB into filaments regulates the subcellular arrangement of a group of enzymes that synthesizes the peptidoglycan (PG) layer. However, it is still unknown how polymerization of MreB determines the rod shape of bacterial cells. Regulatory factor(s) are likely to be involved in controlling the function and dynamics of MreB. We isolated suppressor mutations to partially recover the rod shape in rodZ deletion mutants and found that some of the suppressor mutations occurred in mreB. All of the mreB mutations were in or in the vicinity of domain IA of MreB. Those mreB mutations changed the property of MreB filaments in vivo. In addition, suppressor mutations were found in the periplasmic regions in PBP2 and RodA, encoded by mrdA and mrdB genes. Similar to MreB and RodZ, PBP2 and RodA are pivotal to the cell wall elongation process. Thus, we found that mutations in domain IA of MreB and in the periplasmic domain of PBP2 and RodA can restore growth and rod shape to ΔrodZ cells, possibly by changing the requirements of MreB in the process.
巻・号 87(5)
ページ 1029-44
公開日 2013-3-1
DOI 10.1111/mmi.12148
PMID 23301723
PMC PMC3599482
MeSH Cytoskeletal Proteins / deficiency* Cytoskeletal Proteins / genetics Down-Regulation* Escherichia coli / cytology* Escherichia coli / genetics Escherichia coli / metabolism* Escherichia coli Proteins / genetics* Escherichia coli Proteins / metabolism Gene Deletion Gene Expression Regulation, Bacterial Membrane Proteins / genetics* Membrane Proteins / metabolism Penicillin-Binding Proteins / genetics* Penicillin-Binding Proteins / metabolism Suppression, Genetic*
IF 3.418
WOS 分野 MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) anti-MreB