論文 - 詳細
| RRC ID | 35770 |
|---|---|
| 著者 | Saka K, Tadenuma M, Nakade S, Tanaka N, Sugawara H, Nishikawa K, Ichiyoshi N, Kitagawa M, Mori H, Ogasawara N, Nishimura A. |
| タイトル | A complete set of Escherichia coli open reading frames in mobile plasmids facilitating genetic studies. |
| ジャーナル | DNA Res |
| Abstract |
To facilitate genetic studies of Escherichia coli, we constructed a complete set of mobile plasmid clones of intact open reading frames (ORFs). Their expression is strictly controlled by Ptac / lacI(q). The plasmids carrying each ORF were introduced into an F+ recA strain and stored in 96-well microtiter plates. In this way, 96 clones can be transferred simultaneously to F- bacteria using the conjugative system. This provides a convenient procedure for systematic identification of ORFs that suppress or complement mutations. We created two types of clone sets: the original set contained individual clones in 45 microtiter plates, and a second set contained pools of 48 clones stored in a single microtiter plate. Using these clone sets, we have identified 403 genes that can correct in trans the temperature-sensitive defect of cell division mutants, which would suggest multiple global regulators for bacterial cell division. |
| 巻・号 | 12(1) |
| ページ | 63-8 |
| 公開日 | 2005-2-28 |
| DOI | 10.1093/dnares/12.1.63 |
| PMID | 16106753 |
| MeSH | Base Sequence Cell Division / genetics Cloning, Molecular Escherichia coli / genetics* Genes, Bacterial Genetic Complementation Test / methods Models, Genetic Molecular Sequence Data Open Reading Frames / genetics* Plasmids* |
| IF | 4.009 |
| 引用数 | 62 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Mobile plasmid Collection |