RRC ID 35773
Author Shibahara S, Yoshizawa M, Suzuki H, Takeda K, Meguro K, Endo K.
Title Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation.
Journal J Biochem
Abstract We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hemin or by heat shock (42 degrees C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation.
Volume 113(2)
Pages 214-8
Published 1993-2-1
DOI 10.1093/oxfordjournals.jbchem.a124028
PMID 8468327
MeSH Cell Line DNA Gene Expression Regulation, Enzymologic* HeLa Cells Heme Oxygenase (Decyclizing) / genetics* Humans Isoenzymes / genetics*
IF 2.476
Times Cited 178
DNA material pHHO2-1 (RDB01671)