Reference - Detail
| RRC ID | 35773 |
|---|---|
| Author | Shibahara S, Yoshizawa M, Suzuki H, Takeda K, Meguro K, Endo K. |
| Title | Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. |
| Journal | J Biochem |
| Abstract |
We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hemin or by heat shock (42 degrees C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation. |
| Volume | 113(2) |
| Pages | 214-8 |
| Published | 1993-2-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a124028 |
| PMID | 8468327 |
| MeSH | Cell Line DNA Gene Expression Regulation, Enzymologic* HeLa Cells Heme Oxygenase (Decyclizing) / genetics* Humans Isoenzymes / genetics* |
| IF | 2.476 |
| Times Cited | 178 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pHHO2-1 (RDB01671) |