| RRC ID |
35773
|
| Author |
Shibahara S, Yoshizawa M, Suzuki H, Takeda K, Meguro K, Endo K.
|
| Title |
Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation.
|
| Journal |
J Biochem
|
| Abstract |
We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hemin or by heat shock (42 degrees C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation.
|
| Volume |
113(2)
|
| Pages |
214-8
|
| Published |
1993-2-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a124028
|
| PMID |
8468327
|
| MeSH |
Cell Line
DNA
Gene Expression Regulation, Enzymologic*
HeLa Cells
Heme Oxygenase (Decyclizing) / genetics*
Humans
Isoenzymes / genetics*
|
| IF |
2.476
|
| Times Cited |
178
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| DNA material |
pHHO2-1 (RDB01671) |
