論文 - 詳細
| RRC ID | 35773 |
|---|---|
| 著者 | Shibahara S, Yoshizawa M, Suzuki H, Takeda K, Meguro K, Endo K. |
| タイトル | Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. |
| ジャーナル | J Biochem |
| Abstract |
We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hemin or by heat shock (42 degrees C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation. |
| 巻・号 | 113(2) |
| ページ | 214-8 |
| 公開日 | 1993-2-1 |
| DOI | 10.1093/oxfordjournals.jbchem.a124028 |
| PMID | 8468327 |
| MeSH | Cell Line DNA Gene Expression Regulation, Enzymologic* HeLa Cells Heme Oxygenase (Decyclizing) / genetics* Humans Isoenzymes / genetics* |
| IF | 2.476 |
| 引用数 | 178 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pHHO2-1 (RDB01671) |