RRC ID 35775
Author Yoshida T, Biro P, Cohen T, Müller RM, Shibahara S.
Title Human heme oxygenase cDNA and induction of its mRNA by hemin.
Journal Eur J Biochem
Abstract Hemin treatment increased both activity and mRNA level of heme oxygenase in human macrophages. Using poly(A)-rich RNA prepared from human macrophages treated with hemin, we have constructed a cDNA library in the Okayama-Berg vector. The human heme oxygenase cDNA was isolated by screening this library with a rat cDNA and was subjected to nucleotide sequence analysis. The deduced human heme oxygenase is composed of 288 amino acids with a molecular mass of 32,800 Da. The homology in amino acid sequences between rat and human heme oxygenase is 80%. Like rat heme oxygenase, human enzyme has a putative membrane segment at its carboxyl terminus, which is probably essential for the insertion of heme oxygenase into endoplasmic reticulum. Both rat and human heme oxygenase have no cysteine residues. Recently we have shown that rat heme oxygenase is a heat-shock protein [J. Biol. Chem. 262, 12889-12892 (1987)], and therefore we examined the effects of heat treatment on the induction of heme oxygenase in human macrophages and glioma cells. In contrast to hemin treatment, heat treatment had no apparent effects in either human cell line on the activity of heme oxygenase and its mRNA levels. These results suggest that human heme oxygenase may not be a heat-shock protein.
Volume 171(3)
Pages 457-61
Published 1988-2-1
DOI 10.1111/j.1432-1033.1988.tb13811.x
PMID 3345742
MeSH Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA / analysis* DNA / isolation & purification Enzyme Induction / drug effects Glioma / enzymology Heat-Shock Proteins / isolation & purification Heme / analogs & derivatives* Heme Oxygenase (Decyclizing) / genetics* Hemin / pharmacology* Humans Macrophages / metabolism Mixed Function Oxygenases / genetics* Molecular Sequence Data RNA, Messenger / isolation & purification* Rats
Times Cited 291
DNA material pHHO1 (RDB01207)