Reference - Detail
| RRC ID | 35791 |
|---|---|
| Author | Ohnishi T, Shirane M, Hashimoto Y, Saita S, Nakayama KI. |
| Title | Identification and characterization of a neuron-specific isoform of protrudin. |
| Journal | Genes Cells |
| Abstract |
Protrudin is a membrane protein that regulates polarized vesicular transport. Now, we have identified a novel isoform of protrudin (protrudin-L) that contains an additional seven amino acids between the FFAT motif and the coiled-coil domain compared with the conventional isoform (protrudin-S) as a result of alternative splicing of a microexon (exon L). Protrudin-L mRNA was found to be mostly restricted to the central nervous system in mice, whereas protrudin-S mRNA was detected in all tissues examined. With the use of a splicing reporter minigene that produces two distinct fluorescent proteins in a manner dependent on the splicing pattern of protrudin transcripts, we found that most neurons express protrudin-L, whereas astrocytes express both protrudin isoforms and oligodendrocytes express only protrudin-S. Protrudin-L associated to a greater extent with vesicle-associated membrane protein-associated protein (VAP) than protrudin-S. Expression of protrudin-L in hippocampal neurons of protrudin-deficient mice also promoted neurite outgrowth more efficiently than protrudin-S. Our results suggest that protrudin-L is a neuron-specific protrudin isoform that promotes axonal elongation and contributes to the establishment of neuronal polarity. |
| Volume | 19(2) |
| Pages | 97-111 |
| Published | 2014-2-1 |
| DOI | 10.1111/gtc.12109 |
| PMID | 24251978 |
| MeSH | Alternative Splicing Animals Cell Line, Tumor Mice Mice, Inbred C57BL Molecular Sequence Data Neurons / metabolism* Organ Specificity Protein Isoforms / genetics Protein Isoforms / metabolism R-SNARE Proteins / genetics R-SNARE Proteins / metabolism* Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 1.655 |
| Times Cited | 13 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| Total number of mentions | 0 |
| Resource | |
| DNA material | CSII-CMV-MCS (RDB04377) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394). |