RRC ID 3595
Author Kindt KS, Viswanath V, Macpherson L, Quast K, Hu H, Patapoutian A, Schafer WR.
Title Caenorhabditis elegans TRPA-1 functions in mechanosensation.
Journal Nat. Neurosci.
Abstract Members of the transient receptor potential (TRP) ion channel family mediate diverse sensory transduction processes in both vertebrates and invertebrates. In particular, members of the TRPA subfamily have distinct thermosensory roles in Drosophila, and mammalian TRPA1 is postulated to have a function in noxious cold sensation and mechanosensation. Here we show that mutations in trpa-1, the C. elegans ortholog of mouse Trpa1, confer specific defects in mechanosensory behaviors related to nose-touch responses and foraging. trpa-1 is expressed and functions in sensory neurons required for these mechanosensory behaviors, and contributes to neural responses of these cells to touch, particularly after repeated mechanical stimulation. Furthermore, mechanical pressure can activate C. elegans TRPA-1 heterologously expressed in mammalian cells. Collectively, these data demonstrate that trpa-1 encodes an ion channel that can be activated in response to mechanical pressure and is required for mechanosensory neuron function, suggesting a possible role in mechanosensory transduction or modulation.
Volume 10(5)
Pages 568-77
Published 2007-5
DOI 10.1038/nn1886
PII nn1886
PMID 17450139
MeSH Animals Animals, Genetically Modified CHO Cells Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Cricetinae Cricetulus Gene Expression / physiology Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Head Movements / physiology Mechanoreceptors / physiology* Mechanotransduction, Cellular / physiology* Membrane Potentials / genetics Mice Models, Biological Molecular Sequence Data Mutation / physiology Neurons, Afferent / physiology Patch-Clamp Techniques / methods Reflex / genetics Touch / physiology* Transfection Transient Receptor Potential Channels / genetics Transient Receptor Potential Channels / physiology*
IF 21.126
Times Cited 103
C.elegans tm1402