| RRC ID |
35
|
| 著者 |
Seki T, Matsubayashi H, Amano T, Kitada K, Serikawa T, Sakai N, Sasa M.
|
| タイトル |
Adenoviral gene transfer of aspartoacylase into the tremor rat, a genetic model of epilepsy, as a trial of gene therapy for inherited epileptic disorder.
|
| ジャーナル |
Neurosci Lett
|
| Abstract |
The tremor rat (tm/tm) is a genetic model of epilepsy that exhibits absence-like seizures characterized by 5-7 Hz spike-wave-like complexes in cortical and hippocampal electroencephalograms (EEGs). A deletion of the aspartoacylase (ASPA) gene and resultant high levels of N-acetyl-aspartate (NAA) in the brain have been found in tremor rats. We attempted to determine whether gene transfer of ASPA inhibited absence-like seizures in tremor rats using recombinant adenovirus. Recombinant adenovirus (5x10(7) pfu) carrying the rat ASPA gene (AxASPA) or beta-galactosidase gene (AxLacZ), as a control virus, was intracerebroventricularly administered to premature tremor rats aged 7 weeks. Cortical and hippocampal EEG were recorded with chronically implanted electrodes before and after viral administration. The absence-like seizures were increased in AxLacZ-administered control rats with age. However, the increase was significantly inhibited in AxASPA-administered rats at 1 week after treatment. These results suggest that gene transfer of ASPA is effective in inhibiting the generation of absence-like seizures, probably by reducing the NAA level.
|
| 巻・号 |
328(3)
|
| ページ |
249-52
|
| 公開日 |
2002-8-16
|
| DOI |
10.1016/s0304-3940(02)00522-0
|
| PII |
S0304394002005220
|
| PMID |
12147318
|
| MeSH |
Adenoviridae / genetics
Amidohydrolases / genetics*
Animals
Disease Models, Animal
Epilepsy, Absence / genetics
Female
Gene Transfer Techniques
Genetic Therapy*
Male
Rats
Time Factors
Tremor / genetics*
Tremor / therapy*
|
| IF |
2.274
|
| 引用数 |
15
|
|
WOS 分野
|
NEUROSCIENCES
|
| リソース情報 |
| ラット |
SER/Kyo(strainID=28) |
