RRC ID 36032
Author Shirayama R, Shoji M, Sriwilaijaroen N, Hiramatsu H, Suzuki Y, Kuzuhara T.
Title Inhibition of PA endonuclease activity of influenza virus RNA polymerase by Kampo medicines.
Journal Drug Discov Ther
Abstract To find a novel influenza inhibitor targeting the endonuclease activity of influenza A virus polymerase acidic protein (PA), which is essential for the acquisition of primers for viral mRNA transcription, seven Kampo extracts were tested in vitro for their ability to inhibit endonuclease activity of the recombinant PA protein that was expressed and purified from Escherichia coli. The Kampo medicines Kakkonto, Shosaikoto, Saikokeishito, Keishito, Maobushisaishinto, and Maoto, but not Chikujountanto, inhibited PA endonuclease activity in a dose-dependent manner. Our results indicate that Kampo medicines are good sources providing a structural lead for optimization of an influenza endonuclease inhibitor.
Volume 10(2)
Pages 109-13
Published 2016-1-1
DOI 10.5582/ddt.2016.01010
PMID 26902927
MeSH Dose-Response Relationship, Drug Drugs, Chinese Herbal / pharmacology* Enzyme Inhibitors / pharmacology* Escherichia coli / metabolism Influenza A virus / enzymology Medicine, Kampo* Plasmids / genetics RNA, Messenger / drug effects RNA-Dependent RNA Polymerase / antagonists & inhibitors* RNA-Dependent RNA Polymerase / genetics Recombinant Proteins / chemistry Viral Proteins / antagonists & inhibitors* Viral Proteins / genetics
Times Cited 4
DNA material pBMSA-PA (RDB01971)