| RRC ID |
3607
|
| Author |
Mukhopadhyay S, Lu Y, Qin H, Lanjuin A, Shaham S, Sengupta P.
|
| Title |
Distinct IFT mechanisms contribute to the generation of ciliary structural diversity in C. elegans.
|
| Journal |
EMBO J
|
| Abstract |
Individual cell types can elaborate morphologically diverse cilia. Cilia are assembled via intraflagellar transport (IFT) of ciliary precursors; however, the mechanisms that generate ciliary diversity are unknown. Here, we examine IFT in the structurally distinct cilia of the ASH/ASI and the AWB chemosensory neurons in Caenorhabditis elegans, enabling us to compare IFT in specific cilia types. We show that unlike in the ASH/ASI cilia, the OSM-3 kinesin moves independently of the kinesin-II motor in the AWB cilia. Although OSM-3 is essential to extend the distal segments of the ASH/ASI cilia, it is not required to build the AWB distal segments. Mutations in the fkh-2 forkhead domain gene result in AWB-specific defects in ciliary morphology, and FKH-2 regulates kinesin-II subunit gene expression specifically in AWB. Our results suggest that cell-specific regulation of IFT contributes to the generation of ciliary diversity, and provide insights into the networks coupling the acquisition of ciliary specializations with other aspects of cell fate.
|
| Volume |
26(12)
|
| Pages |
2966-80
|
| Published |
2007-6-20
|
| DOI |
10.1038/sj.emboj.7601717
|
| PII |
7601717
|
| PMID |
17510633
|
| PMC |
PMC1894762
|
| MeSH |
Animals
Animals, Genetically Modified
Biological Transport
Caenorhabditis elegans / cytology*
Caenorhabditis elegans Proteins / physiology
Cilia / ultrastructure*
Kinesins / physiology
Microscopy, Electron
Mutation
Olfactory Receptor Neurons / ultrastructure
|
| IF |
9.889
|
| Times Cited |
65
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm324 |
