Reference - Detail
| RRC ID | 3608 |
|---|---|
| Author | Mullen GP, Mathews EA, Vu MH, Hunter JW, Frisby DL, Duke A, Grundahl K, Osborne JD, Crowell JA, Rand JB. |
| Title | Choline transport and de novo choline synthesis support acetylcholine biosynthesis in Caenorhabditis elegans cholinergic neurons. |
| Journal | Genetics |
| Abstract |
The cho-1 gene in Caenorhabditis elegans encodes a high-affinity plasma-membrane choline transporter believed to be rate limiting for acetylcholine (ACh) synthesis in cholinergic nerve terminals. We found that CHO-1 is expressed in most, but not all cholinergic neurons in C. elegans. cho-1 null mutants are viable and exhibit mild deficits in cholinergic behavior; they are slightly resistant to the acetylcholinesterase inhibitor aldicarb, and they exhibit reduced swimming rates in liquid. cho-1 mutants also fail to sustain swimming behavior; over a 33-min time course, cho-1 mutants slow down or stop swimming, whereas wild-type animals sustain the initial rate of swimming over the duration of the experiment. A functional CHO-1GFP fusion protein rescues these cho-1 mutant phenotypes and is enriched at cholinergic synapses. Although cho-1 mutants clearly exhibit defects in cholinergic behaviors, the loss of cho-1 function has surprisingly mild effects on cholinergic neurotransmission. However, reducing endogenous choline synthesis strongly enhances the phenotype of cho-1 mutants, giving rise to a synthetic uncoordinated phenotype. Our results indicate that both choline transport and de novo synthesis provide choline for ACh synthesis in C. elegans cholinergic neurons. |
| Volume | 177(1) |
| Pages | 195-204 |
| Published | 2007-9-1 |
| DOI | 10.1534/genetics.107.074120 |
| PII | genetics.107.074120 |
| PMID | 17603106 |
| PMC | PMC2013710 |
| MeSH | Acetylcholine / biosynthesis* Adaptation, Physiological Animals Animals, Genetically Modified Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins Choline / pharmacokinetics* Fluorescent Antibody Technique Membrane Transport Proteins / genetics Membrane Transport Proteins / metabolism* Neurons / cytology Neurons / metabolism* Synapses / metabolism* Synaptic Transmission Tissue Distribution |
| IF | 4.015 |
| Times Cited | 15 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm373 |