Reference - Detail
|Author||Kohno K, Takahashi H, Endo TR, Matsuo H, Shiwaku K, Morita E.|
|Title||Characterization of a hypoallergenic wheat line lacking ω-5 gliadin.|
BACKGROUND:There is no curative treatment for wheat-dependent exercise-induced anaphylaxis (WDEIA). ω-5 Gliadin is one of the dominant allergens affecting WDEIA patients. The use of ω-5 gliadin-free wheat flour in the regular diet is considered one of the prophylactic approaches against the elicitation of allergic symptoms and sensitization to ω-5 gliadin. We sought to find hypoallergenic bread wheat (or common wheat) that lacked the genes encoding ω-5 gliadin and to evaluate its in vitro allergenicity. We also aimed to evaluate the sensitization ability of one of the selected hypoallergenic wheat lines by using a possible animal model of wheat allergy.
METHODS:We screened the deletion lines of bread wheat by western blotting to ascertain common wheat lines lacking the ω-5 gliadin locus. The deletion lines we used have partial deficiency of chromosome 1B (Endo and Gill, 1996). To assess sensitization ability of gluten from the selected deletion line, guinea pigs were fed with either the gluten from the selected deletion line or commercially available gluten, and allergic score was evaluated after challenging the same gluten preparations.
RESULTS:We found that a deletion line 1BS-18 had the least deficiency of chromosome 1B among the deletion stocks lacking the ω-5 gliadin locus. The challenge test using the guinea pigs revealed that the symptoms induced by application of the 1BS-18 gluten were much less than that of commercially available gluten.
CONCLUSIONS:The deletion line 1BS-18, which lacked the ω-5 gliadin locus, is likely to have a low sensitization capacity in the guinea pig. The use of the wheat products of the 1BS-18 line in daily life may provide a feasible solution for the onset of wheat allergy.
|MeSH||Allergens / administration & dosage Allergens / immunology* Animals Antibodies / immunology Antigens, Plant / genetics* Antigens, Plant / immunology* Disease Models, Animal Epitopes / chemistry Epitopes / immunology Flour Gliadin / genetics* Gliadin / immunology* Guinea Pigs Immunoglobulin E / immunology Immunoglobulin G / immunology Peptides / immunology Plants, Genetically Modified Triticum / adverse effects* Triticum / genetics* Wheat Hypersensitivity / immunology*|
|WOS Category||IMMUNOLOGY ALLERGY|
|Wheat||LPGKU2269 LPGKU0043 LPGKU0044 LPGKU0045 LPGKU0046 LPGKU0047 LPGKU0048 LPGKU1010 LPGKU1027 LPGKU1030 LPGKU1039 LPGKU1040 LPGKU1043 LPGKU1044 LPGKU1045|