RRC ID 36133
Author Cuesta-Marcos A, Muñoz-Amatriaín M, Filichkin T, Karsai I, Trevaskis B, Yasuda S, Hayes P, Sato K.
Title The Relationships between Development and Low Temperature Tolerance in Barley Near Isogenic Lines Differing for Flowering Behavior.
Journal Plant Cell Physiol
Abstract Flowering time, vernalization requirement, photoperiod sensitivity and low temperature tolerance are key traits in the Triticeae. We characterized a set of isogenic genetic stocks-representing single and pairwise substitutions of spring alleles at the VRN-H1, VRN-H2 and VRN-H3 loci in a winter barley background-at the structural, functional and phenotypic levels. High density mapping with reference to the barley genome sequence confirmed that in all cases target VRN alleles were present in the near isogenic lines (NILs) and allowed estimates of introgression size (at the genetic and physical levels) and gene content. Expression data corroborated the structural and phenotypic results. The latter confirmed that substitution of a spring allele at any of the VRN loci is sufficient to eliminate vernalization requirement. There was no significant change in low temperature tolerance with substitution of a spring allele at VRN-H2, but there were significant losses in cold tolerance with substitutions at VRN-H1 and VRN-H3. Reductions in cold tolerance are ascribed to an accelerated transition from the vegetative to reproductive state. The set of NILs will be a rich resource for understanding the genetics of vernalization, low temperature tolerance and other traits encoded/regulated by genes within the introgressed intervals.
Volume 56(12)
Pages 2312-24
Published 2015-12-1
DOI 10.1093/pcp/pcv147
PII pcv147
PMID 26443377
MeSH Adaptation, Physiological* / genetics Alleles Cold Temperature* Flowers / physiology* Freezing Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Genotype Hordeum / genetics Hordeum / growth & development* Hordeum / physiology* Inbreeding Reproduction Time Factors
IF 4.062
Times Cited 14
Barley Barley seed samples