RRC ID 36149
Author Kawamoto M, Koga H, Kiuchi T, Shoji K, Sugano S, Shimada T, Suzuki Y, Katsuma S.
Title Sexually biased transcripts at early embryonic stages of the silkworm depend on the sex chromosome constitution.
Journal Gene
Abstract In the silkworm, Bombyx mori, females are heterogametic (WZ) whereas males have two Z chromosomes. Femaleness of B. mori is determined by the presence of the W chromosome, suggesting that there is a dominant feminizing gene on this chromosome. Recently, by transcriptome analysis of B. mori embryos, we discovered that a single W-chromosome-derived PIWI-interacting RNA (piRNA) is the long-sought primary determinant of femaleness in B. mori. However, sexual bias in the transcriptome of B. mori early embryos has not yet been well characterized. Using deep sequencing data from molecularly sexed RNA of B. mori embryos, we identified and characterized 157 transcripts that are statistically differentially expressed between male and female early embryos. Most of the female-biased transcripts were transposons or repeat sequences that are produced presumably from the W chromosome. Bioinformatic analysis revealed that these repetitive sequences are piRNA precursors. In contrast, male-biased genes were frequently transcribed from the Z chromosome, suggesting that dosage compensation in Z-linked genes does not occur or is incomplete at early embryonic stages. Our analysis has drawn a picture of a global landscape of sexually biased transcriptome during early B. mori embyogenesis and has suggested for the first time that most sexually biased embryonic transcripts depend on sex chromosomes.
Volume 560(1)
Pages 50-6
Published 2015-4-10
DOI 10.1016/j.gene.2015.01.036
PII S0378-1119(15)00066-9
PMID 25615878
MeSH Animals Bombyx / embryology* Bombyx / genetics* Chromosomes, Insect Embryo, Nonmammalian Embryonic Development / genetics* Female Gene Expression Profiling Insect Proteins / genetics Male Microarray Analysis RNA, Messenger / genetics* Sex Characteristics Sex Chromosomes*
IF 2.498
Times Cited 2
WOS Category GENETICS & HEREDITY
Resource
Silkworms