論文 - 詳細
| RRC ID | 36154 |
|---|---|
| 著者 | Li W, Gong M, Shu R, Li X, Gao J, Meng Y. |
| タイトル | Molecular and enzymatic characterization of two enzymes BmPCD and BmDHPR involving in the regeneration pathway of tetrahydrobiopterin from the silkworm Bombyx mori. |
| ジャーナル | Comp Biochem Physiol B Biochem Mol Biol |
| Abstract |
Tetrahydrobiopterin (BH4) is an essential cofactor of aromatic amino acid hydroxylases and nitric oxide synthase so that BH4 plays a key role in many biological processes. BH4 deficiency is associated with numerous metabolic syndromes and neuropsychological disorders. BH4 concentration in mammals is maintained through a de novo synthesis pathway and a regeneration pathway. Previous studies showed that the de novo pathway of BH4 is similar between insects and mammals. However, knowledge about the regeneration pathway of BH4 (RPB) is very limited in insects. Several mutants in the silkworm Bombyx mori have been approved to be associated with BH4 deficiency, which are good models to research on the RPB in insects. In this study, homologous genes encoding two enzymes, pterin-4a-carbinolamine dehydratase (PCD) and dihydropteridine reductase (DHPR) involving in RPB have been cloned and identified from B. mori. Enzymatic activity of DHPR was found in the fat body of wild type silkworm larvae. Together with the transcription profiles, it was indicated that BmPcd and BmDhpr might normally act in the RPB of B. mori and the expression of BmDhpr was activated in the brain and sexual glands while BmPcd was expressed in a wider special pattern when the de novo pathway of BH4 was lacked in lemon. Biochemical analyses showed that the recombinant BmDHPR exhibited high enzymatic activity and more suitable parameters to the coenzyme of NADH in vitro. The results in this report give new information about the RPB in B. mori and help in better understanding insect BH4 biosynthetic networks. |
| 巻・号 | 186 |
| ページ | 20-7 |
| 公開日 | 2015-8-1 |
| DOI | 10.1016/j.cbpb.2015.04.006 |
| PII | S1096-4959(15)00064-0 |
| PMID | 25899859 |
| MeSH | Amino Acid Sequence Animals Biopterin / analogs & derivatives* Biopterin / metabolism Bombyx / chemistry Bombyx / enzymology* Bombyx / physiology* Dihydropteridine Reductase / chemistry Dihydropteridine Reductase / genetics Dihydropteridine Reductase / metabolism* Enzyme Activation Hydro-Lyases / chemistry Hydro-Lyases / genetics Hydro-Lyases / metabolism* Larva / physiology* Metabolic Networks and Pathways Molecular Sequence Data RNA, Messenger / metabolism Regeneration |
| IF | 2.219 |
| 引用数 | 3 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY ZOOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50 a60 a65 |