論文 - 詳細
RRC ID | 36159 |
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著者 | Lenhart KF, DiNardo S. |
タイトル | Somatic cell encystment promotes abscission in germline stem cells following a regulated block in cytokinesis. |
ジャーナル | Dev Cell |
Abstract |
In many tissues, the stem cell niche must coordinate behavior across multiple stem cell lineages. How this is achieved is largely unknown. We have identified delayed completion of cytokinesis in germline stem cells (GSCs) as a mechanism that regulates the production of stem cell daughters in the Drosophila testis. Through live imaging, we show that a secondary F-actin ring is formed through regulation of Cofilin activity to block cytokinesis progress after contractile ring disassembly. The duration of this block is controlled by Aurora B kinase. Additionally, we have identified a requirement for somatic cell encystment of the germline in promoting GSC abscission. We suggest that this non-autonomous role promotes coordination between stem cell lineages. These findings reveal the mechanisms by which cytokinesis is inhibited and reinitiated in GSCs and why such complex regulation exists within the stem cell niche. |
巻・号 | 34(2) |
ページ | 192-205 |
公開日 | 2015-7-27 |
DOI | 10.1016/j.devcel.2015.05.003 |
PII | S1534-5807(15)00319-6 |
PMID | 26143993 |
PMC | PMC4519359 |
MeSH | Actin Depolymerizing Factors / metabolism Actins / metabolism Animals Aurora Kinase B / metabolism* Cytokinesis / physiology* Drosophila Drosophila Proteins / metabolism* Germ Cells / cytology* Lim Kinases / metabolism Male Phosphorylation Stem Cell Niche / physiology Stem Cells / cytology* Stem Cells / metabolism Testis / cytology Testis / growth & development rho-Associated Kinases / antagonists & inhibitors rho-Associated Kinases / metabolism |
IF | 10.092 |
引用数 | 23 |
WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
リソース情報 | |
ショウジョウバエ | Traffic Jam Gal4 (DGRC#104055) |