論文 - 詳細
| RRC ID | 36170 |
|---|---|
| 著者 | Laurinyecz B, Péter M, Vedelek V, Kovács AL, Juhász G, Maróy P, Vígh L, Balogh G, Sinka R. |
| タイトル | Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila. |
| ジャーナル | Open Biol |
| Abstract |
Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correct lipid composition during sperm development and show that phosphatidic acid levels are crucial in late stages of spermatogenesis. |
| 巻・号 | 6(1) |
| ページ | 50169 |
| 公開日 | 2016-1-1 |
| DOI | 10.1098/rsob.150169 |
| PII | rsob.150169 |
| PMID | 26791243 |
| PMC | PMC4736822 |
| MeSH | Alleles Animals Diacylglycerol Cholinephosphotransferase Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology* Drosophila melanogaster / physiology* Genes, Insect Infertility, Male / enzymology* Infertility, Male / pathology Lipids / biosynthesis Lipids / chemistry* Male Mitochondria Mutation / genetics Nucleotidyltransferases / metabolism* Phosphatidic Acids / metabolism Phosphatidylinositols / metabolism Phosphotransferases Spermatids / metabolism Spermatids / ultrastructure Spermatogenesis Testis / metabolism |
| IF | 4.931 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | DGRC#201308 |