RRC ID |
36170
|
著者 |
Laurinyecz B, Péter M, Vedelek V, Kovács AL, Juhász G, Maróy P, Vígh L, Balogh G, Sinka R.
|
タイトル |
Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.
|
ジャーナル |
Open Biol
|
Abstract |
Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correct lipid composition during sperm development and show that phosphatidic acid levels are crucial in late stages of spermatogenesis.
|
巻・号 |
6(1)
|
ページ |
50169
|
公開日 |
2016-1-1
|
DOI |
10.1098/rsob.150169
|
PII |
rsob.150169
|
PMID |
26791243
|
PMC |
PMC4736822
|
MeSH |
Alleles
Animals
Diacylglycerol Cholinephosphotransferase
Drosophila Proteins / metabolism*
Drosophila melanogaster / enzymology*
Drosophila melanogaster / physiology*
Genes, Insect
Infertility, Male / enzymology*
Infertility, Male / pathology
Lipids / biosynthesis
Lipids / chemistry*
Male
Mitochondria
Mutation / genetics
Nucleotidyltransferases / metabolism*
Phosphatidic Acids / metabolism
Phosphatidylinositols / metabolism
Phosphotransferases
Spermatids / metabolism
Spermatids / ultrastructure
Spermatogenesis
Testis / metabolism
|
IF |
4.931
|
引用数 |
12
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ショウジョウバエ |
DGRC#201308 |