RRC ID 36170
著者 Laurinyecz B, Péter M, Vedelek V, Kovács AL, Juhász G, Maróy P, Vígh L, Balogh G, Sinka R.
タイトル Reduced expression of CDP-DAG synthase changes lipid composition and leads to male sterility in Drosophila.
ジャーナル Open Biol
Abstract Drosophila spermatogenesis is an ideal system to study the effects of changes in lipid composition, because spermatid elongation and individualization requires extensive membrane biosynthesis and remodelling. The bulk of transcriptional activity is completed with the entry of cysts into meiotic division, which makes post-meiotic stages of spermatogenesis very sensitive to even a small reduction in gene products. In this study, we describe the effect of changes in lipid composition during spermatogenesis using a hypomorphic male sterile allele of the Drosophila CDP-DAG synthase (CdsA) gene. We find that the CdsA mutant shows defects in spermatid individualization and enlargement of mitochondria and the axonemal sheath of the spermatids. Furthermore, we could genetically rescue the male sterile phenotype by overexpressing Phosphatidylinositol synthase (dPIS) in a CdsA mutant background. The results of lipidomic and genetic analyses of the CdsA mutant highlight the importance of correct lipid composition during sperm development and show that phosphatidic acid levels are crucial in late stages of spermatogenesis.
巻・号 6(1)
ページ 50169
公開日 2016-1-1
DOI 10.1098/rsob.150169
PII rsob.150169
PMID 26791243
PMC PMC4736822
MeSH Alleles Animals Diacylglycerol Cholinephosphotransferase Drosophila Proteins / metabolism* Drosophila melanogaster / enzymology* Drosophila melanogaster / physiology* Genes, Insect Infertility, Male / enzymology* Infertility, Male / pathology Lipids / biosynthesis Lipids / chemistry* Male Mitochondria Mutation / genetics Nucleotidyltransferases / metabolism* Phosphatidic Acids / metabolism Phosphatidylinositols / metabolism Phosphotransferases Spermatids / metabolism Spermatids / ultrastructure Spermatogenesis Testis / metabolism
IF 4.931
引用数 12
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ショウジョウバエ DGRC#201308