論文 - 詳細
| RRC ID | 36217 |
|---|---|
| 著者 | Kanno T, Takekawa D, Miyakawa Y. |
| タイトル | Analysis of the essentiality of ROM2 genes in the pathogenic yeasts Candida glabrata and Candida albicans using temperature-sensitive mutants. |
| ジャーナル | J Appl Microbiol |
| Abstract |
AIMS:To analyse the essentiality of the ROM2 genes originating from the pathogenic yeasts Candida glabrata and Candida albicans by using temperature-sensitive (ts) mutants. METHODS AND RESULTS:Based on the general concepts that ts mutations are generated by virtue of point mutation within essential genes, we have previously established a novel method (termed 'ETS system' for screening and identification of essential genes using ts mutants of C. glabrata). According to this ETS system, the present study successfully identified a putative C. glabrata ROM2 homologue as an essential gene that complements its point mutation (Cys-1275/Tyr substitution). The C. albicans ROM2 mutant (Cys-1281/Tyr), constructed patterned after this point mutation, also displayed ts phenotype. Both ts mutants recovered colony-forming ability, with concomitant suppression of lysis phenotype, at the elevated temperature in the presence of 1 mol l(-1) sorbitol as an osmotic stabilizer. Sequence alignment revealed that human genome possesses relatively low homology against Rom2 homologues, which are highly conserved among yeast species. CONCLUSIONS:ROM2 genes of C. glabrata and C. albicans are essential for viability, probably involved in cell wall integrity. SIGNIFICANCE AND IMPACT OF THE STUDY:ROM2 genes essential for both Candida species may be a potentially useful antifungal targets from chemotherapeutic viewpoint. |
| 巻・号 | 118(4) |
| ページ | 851-63 |
| 公開日 | 2015-4-1 |
| DOI | 10.1111/jam.12745 |
| PMID | 25604069 |
| MeSH | Amino Acid Sequence Candida albicans / chemistry Candida albicans / genetics* Candida albicans / metabolism Candida albicans / pathogenicity Candida glabrata / chemistry Candida glabrata / genetics* Candida glabrata / metabolism Candida glabrata / pathogenicity Candidiasis / microbiology* Fungal Proteins / chemistry Fungal Proteins / genetics* Fungal Proteins / metabolism Humans Molecular Sequence Data Mutation Sequence Alignment Temperature Virulence |
| IF | 3.066 |
| 引用数 | 2 |
| WOS 分野 | BIOTECHNOLOGY & APPLIED MICROBIOLOGY MICROBIOLOGY |
| リソース情報 | |
| 酵母 | BY24967 |