RRC ID 36217
Author Kanno T, Takekawa D, Miyakawa Y.
Title Analysis of the essentiality of ROM2 genes in the pathogenic yeasts Candida glabrata and Candida albicans using temperature-sensitive mutants.
Journal J Appl Microbiol
Abstract AIMS:To analyse the essentiality of the ROM2 genes originating from the pathogenic yeasts Candida glabrata and Candida albicans by using temperature-sensitive (ts) mutants.
METHODS AND RESULTS:Based on the general concepts that ts mutations are generated by virtue of point mutation within essential genes, we have previously established a novel method (termed 'ETS system' for screening and identification of essential genes using ts mutants of C. glabrata). According to this ETS system, the present study successfully identified a putative C. glabrata ROM2 homologue as an essential gene that complements its point mutation (Cys-1275/Tyr substitution). The C. albicans ROM2 mutant (Cys-1281/Tyr), constructed patterned after this point mutation, also displayed ts phenotype. Both ts mutants recovered colony-forming ability, with concomitant suppression of lysis phenotype, at the elevated temperature in the presence of 1 mol l(-1) sorbitol as an osmotic stabilizer. Sequence alignment revealed that human genome possesses relatively low homology against Rom2 homologues, which are highly conserved among yeast species.
CONCLUSIONS:ROM2 genes of C. glabrata and C. albicans are essential for viability, probably involved in cell wall integrity.
SIGNIFICANCE AND IMPACT OF THE STUDY:ROM2 genes essential for both Candida species may be a potentially useful antifungal targets from chemotherapeutic viewpoint.
Volume 118(4)
Pages 851-63
Published 2015-4-1
DOI 10.1111/jam.12745
PMID 25604069
MeSH Amino Acid Sequence Candida albicans / chemistry Candida albicans / genetics* Candida albicans / metabolism Candida albicans / pathogenicity Candida glabrata / chemistry Candida glabrata / genetics* Candida glabrata / metabolism Candida glabrata / pathogenicity Candidiasis / microbiology* Fungal Proteins / chemistry Fungal Proteins / genetics* Fungal Proteins / metabolism Humans Molecular Sequence Data Mutation Sequence Alignment Temperature Virulence
IF 3.066
Times Cited 2
Yeast BY24967