Reference - Detail
| RRC ID | 36234 |
|---|---|
| Author | Kravchuk OI, Mikhailov VS, Savitsky MY. |
| Title | [A Simple and Efficient Method of Inducing Targeted Deletions in the Drosophila Genome]. |
| Journal | Genetika |
| Abstract |
Deletion mutagenesis is one of the most efficient approaches to studying gene function. However, conventional methods of inducing targeted mutations in the drosophila genome are time- and labor-consuming. This work proposes a new, simple, and effective method of producing drosophila mutants with gene deletions. The method involves the insertion of I-Scel and I-CreI recognition sites and a fragment homologous to the target sequence into the chromosome region of interest by means of an attB-containing construct, the induction of double-strand DNA breaks by the appropriate meganuclease, and their repair by homologous recombination. The procedure results in a deletion extending from the attP-site to the target locus. A cassette was designed to enable single-step construct production for the deletion of any given genomic region. A set of markers facilitates the selection of recombination events. The efficacy of the proposed technique was confirmed by the induction of a 47-kb deletion containing the qtc gene. |
| Volume | 51(11) |
| Pages | 1325-9 |
| Published | 2015-11-1 |
| PMID | 26845863 |
| MeSH | Animals DNA Breaks* Drosophila melanogaster Gene Deletion* Genome, Insect* Mutagenesis* Recombinational DNA Repair* |
| IF | 0.403 |
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| Total number of mentions | 0 |
| Resource | |
| Drosophila | |