論文 - 詳細
| RRC ID | 36268 |
|---|---|
| 著者 | Inagaki Y, Matsumoto Y, Ishii M, Uchino K, Sezutsu H, Sekimizu K. |
| タイトル | Fluorescence imaging for a noninvasive in vivo toxicity-test using a transgenic silkworm expressing green fluorescent protein. |
| ジャーナル | Sci Rep |
| Abstract |
In drug development, the toxicity of candidate chemicals must be carefully examined in an animal model. Here we developed a live imaging technique using silkworms for a noninvasive toxicity test applicable for drug screening. Injection of carbon tetrachloride, a tissue-injuring chemical, into transgenic silkworms expressing green fluorescent protein (GFP) induced leakage of GFP from the tissues into the hemolymph. The leakage of GFP was suppressed by pre-administration of either cimetidine, a cytochrome P450 inhibitor, or N-acetyl cysteine, a free-radical scavenger. The transgenic silkworm was made transparent by feeding a diet containing chemicals that inhibit uric acid deposition in the epithelial cells. In the transparent silkworms, GFP fluorescence in the fat body could be observed from outside the body. Injection of salicylic acid or iron sulfate, tissue-injuring chemicals, into the transparent silkworms decreased the fluorescence intensity of the GFP in the fat body. These findings suggest that the transparent GFP-expressing silkworm model is useful for evaluating the toxicity of chemicals that induce tissue injury. |
| 巻・号 | 5 |
| ページ | 11180 |
| 公開日 | 2015-6-10 |
| DOI | 10.1038/srep11180 |
| PII | srep11180 |
| PMID | 26061948 |
| PMC | PMC4462092 |
| MeSH | Animals Animals, Genetically Modified Bombyx / drug effects* Bombyx / genetics* Bombyx / metabolism Catalysis Cytochrome P-450 Enzyme System / metabolism Drug Evaluation, Preclinical / methods Gene Expression* Green Fluorescent Proteins / genetics* Green Fluorescent Proteins / metabolism Hemolymph / metabolism Phenotype Toxicity Tests* / methods |
| IF | 3.998 |
| 引用数 | 12 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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