| RRC ID |
36271
|
| 著者 |
Tada M, Tatematsu K, Ishii-Watabe A, Harazono A, Takakura D, Hashii N, Sezutsu H, Kawasaki N.
|
| タイトル |
Characterization of anti-CD20 monoclonal antibody produced by transgenic silkworms (Bombyx mori).
|
| ジャーナル |
MAbs
|
| Abstract |
In response to the successful use of monoclonal antibodies (mAbs) in the treatment of various diseases, systems for expressing recombinant mAbs using transgenic animals or plants have been widely developed. The silkworm (Bombyx mori) is a highly domesticated insect that has recently been used for the production of recombinant proteins. Because of their cost-effective breeding and relatively easy production scale-up, transgenic silkworms show great promise as a novel production system for mAbs. In this study, we established a transgenic silkworm stably expressing a human-mouse chimeric anti-CD20 mAb having the same amino acid sequence as rituximab, and compared its characteristics with rituximab produced by Chinese hamster ovary (CHO) cells (MabThera®). The anti-CD20 mAb produced in the transgenic silkworm showed a similar antigen-binding property, but stronger antibody-dependent cell-mediated cytotoxicity (ADCC) and weaker complement-dependent cytotoxicity (CDC) compared to MabThera. Post-translational modification analysis was performed by peptide mapping using liquid chromatography/mass spectrometry. There was a significant difference in the N-glycosylation profile between the CHO- and the silkworm-derived mAbs, but not in other post-translational modifications including oxidation and deamidation. The mass spectra of the N-glycosylated peptide revealed that the observed biological properties were attributable to the characteristic N-glycan structures of the anti-CD20 mAbs produced in the transgenic silkworms, i.e., the lack of the core-fucose and galactose at the non-reducing terminal. These results suggest that the transgenic silkworm may be a promising expression system for the tumor-targeting mAbs with higher ADCC activity.
|
| 巻・号 |
7(6)
|
| ページ |
1138-50
|
| 公開日 |
2015-1-1
|
| DOI |
10.1080/19420862.2015.1078054
|
| PMID |
26261057
|
| PMC |
PMC4966511
|
| MeSH |
Animals
Animals, Genetically Modified
Antibodies, Monoclonal / genetics
Antibodies, Monoclonal / immunology*
Antibodies, Monoclonal / metabolism
Antibody Specificity / immunology*
Antibody-Dependent Cell Cytotoxicity / immunology
Antigens, CD20 / immunology*
Bombyx / genetics*
CHO Cells
Cell Line, Tumor
Chromatography, Liquid
Complement System Proteins / immunology
Cricetinae
Cricetulus
Cytotoxicity, Immunologic / immunology
Glycosylation
Humans
Mass Spectrometry
Mice
Recombinant Proteins / immunology*
Recombinant Proteins / metabolism
Rituximab / genetics
Rituximab / immunology
Rituximab / metabolism
|
| IF |
4.634
|
| 引用数 |
21
|
|
WOS 分野
|
MEDICINE, RESEARCH & EXPERIMENTAL
|
| リソース情報 |
| カイコ |
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