論文 - 詳細
| RRC ID | 36275 |
|---|---|
| 著者 | Shiomi K, Takasu Y, Kunii M, Tsuchiya R, Mukaida M, Kobayashi M, Sezutsu H, Ichida Takahama M, Mizoguchi A. |
| タイトル | Disruption of diapause induction by TALEN-based gene mutagenesis in relation to a unique neuropeptide signaling pathway in Bombyx. |
| ジャーナル | Sci Rep |
| Abstract |
The insect neuropeptide family FXPRLa, which carries the Phe-Xaa-Pro-Arg-Leu-NH2 sequence at the C-terminus, is involved in many physiological processes. Although ligand-receptor interactions in FXPRLa signaling have been examined using in vitro assays, the correlation between these interactions and in vivo physiological function is unclear. Diapause in the silkworm, Bombyx mori, is thought to be elicited by diapause hormone (DH, an FXPRLa) signaling, which consists of interactions between DH and DH receptor (DHR). Here, we performed transcription activator-like effector nuclease (TALEN)-based mutagenesis of the Bombyx DH-PBAN and DHR genes and isolated the null mutants of these genes in a bivoltine strain. All mutant silkworms were fully viable and showed no abnormalities in the developmental timing of ecdysis or metamorphosis. However, female adults oviposited non-diapause eggs despite diapause-inducing temperature and photoperiod conditions. Therefore, we conclude that DH signaling is essential for diapause induction and consists of highly sensitive and specific interactions between DH and DHR selected during ligand-receptor coevolution in Bombyx mori. |
| 巻・号 | 5 |
| ページ | 15566 |
| 公開日 | 2015-10-26 |
| DOI | 10.1038/srep15566 |
| PII | srep15566 |
| PMID | 26497859 |
| PMC | PMC4620438 |
| MeSH | Amino Acid Sequence Animals Base Sequence Bombyx Female Insect Proteins / genetics Metamorphosis, Biological / genetics Metamorphosis, Biological / physiology* Molecular Sequence Data Mutagenesis* Mutation Neuropeptides / genetics* Receptors, Peptide / genetics* Signal Transduction* |
| IF | 3.998 |
| 引用数 | 14 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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