RRC ID 36277
Author Froschauer A, Kube L, Kegler A, Rieger C, Gutzeit HO.
Title Tunable Protein Stabilization In Vivo Mediated by Shield-1 in Transgenic Medaka.
Journal PLoS One
Abstract Techniques for conditional gene or protein expression are important tools in developmental biology and in the analysis of physiology and disease. On the protein level, the tunable and reversible expression of proteins can be achieved by the fusion of the protein of interest to a destabilizing domain (DD). In the absence of its specific ligand (Shield-1), the protein is degraded by the proteasome. The DD-Shield system has proven to be an excellent tool to regulate the expression of proteins of interests in mammalian systems but has not been applied in teleosts like the medaka. We present the application of the DD-Shield technique in transgenic medaka and show the ubiquitous conditional expression throughout life. Shield-1 administration to the water leads to concentration-dependent induction of a YFP reporter gene in various organs and in spermatogonia at the cellular level.
Volume 10(7)
Pages e0131252
Published 2015-1-1
DOI 10.1371/journal.pone.0131252
PII PONE-D-15-08748
PMID 26148066
PMC PMC4493054
MeSH Animals Animals, Genetically Modified / metabolism* Gene Expression Regulation / drug effects Genes, Reporter / drug effects Ligands Male Morpholines / pharmacology* Oryzias / genetics Oryzias / metabolism* Proteasome Endopeptidase Complex / metabolism Protein Structure, Tertiary Proteins / metabolism*
IF 2.74
Times Cited 2
Medaka FLFII (MT109) STII Hd-rR.Y^HNI (MT191)