論文 - 詳細
| RRC ID | 36328 |
|---|---|
| 著者 | Chen Z, Nohata J, Guo H, Li S, Liu J, Guo Y, Yamamoto K, Kadono-Okuda K, Liu C, Arunkumar KP, Nagaraju J, Zhang Y, Liu S, Labropoulou V, Swevers L, Tsitoura P, Iatrou K, Gopinathan KP, Goldsmith MR, Xia Q, Mita K. |
| タイトル | A comprehensive analysis of the chorion locus in silkmoth. |
| ジャーナル | Sci Rep |
| Abstract |
Despite more than 40 years of intense study, essential features of the silkmoth chorion (eggshell) are still not fully understood. To determine the precise structure of the chorion locus, we performed extensive EST analysis, constructed a bacterial artificial chromosome (BAC) contig, and obtained a continuous genomic sequence of 871,711 base pairs. We annotated 127 chorion genes in two segments interrupted by a 164 kb region with 5 non-chorion genes, orthologs of which were on chorion bearing scaffolds in 4 ditrysian families. Detailed transcriptome analysis revealed expression throughout choriogenesis of most chorion genes originally categorized as "middle", and evidence for diverse regulatory mechanisms including cis-elements, alternative splicing and promoter utilization, and antisense RNA. Phylogenetic analysis revealed multigene family associations and faster evolution of early chorion genes and transcriptionally active pseudogenes. Proteomics analysis identified 99 chorion proteins in the eggshell and micropyle localization of 1 early and 6 Hc chorion proteins. |
| 巻・号 | 5 |
| ページ | 16424 |
| 公開日 | 2015-11-10 |
| DOI | 10.1038/srep16424 |
| PII | srep16424 |
| PMID | 26553298 |
| PMC | PMC4639761 |
| MeSH | Animals Bombyx / genetics* Bombyx / metabolism Chorion* Computational Biology / methods Egg Proteins Egg Shell Expressed Sequence Tags Gene Expression Regulation Gene Library Gene Order High-Throughput Nucleotide Sequencing Molecular Sequence Annotation Phylogeny Promoter Regions, Genetic Proteome Proteomics / methods Quantitative Trait Loci* Transcription, Genetic |
| IF | 3.998 |
| 引用数 | 11 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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| カイコ | |