RRC ID 36333
著者 Horie Y, Shimizu A, Adachi S, Kobayashi T.
タイトル Expression and localization of gonadotropic hormone subunits (Gpa, Fshb, and Lhb) in the pituitary during gonadal differentiation in medaka.
ジャーナル Gen Comp Endocrinol
Abstract To clarify the appearance of and chronological changes in two different gonadotropic hormone (Gth) cells, we examined the dynamics of Gth cells in detail during gonadal differentiation and development in the d-rR strain of medaka (Oryzias latipes). Expression of the sex-determining gene Dmy was evident in gonadal somatic cells at 5 days post-fertilization (dpf). Glycoprotein-α (Gpa)-positive cells first appeared in the pituitary at 4 dpf, regardless of genetic sex, while follicle-stimulating hormone-β (Fshb)-positive cells was detected in XX and XY embryos at 5 and 6 dpf, respectively. In contrast, luteinizing hormone-β (Lhb)-positive cells were observed in both sexes of medaka after 70 days post-hatching (dph). The density of Fshb-positive cells in the pituitary was significantly and transiently higher in XX than in XY fry at 0 dph, and thereafter no significant differences were detected before sexual maturation. In this study, temporal expression of Fshb was observed, indicating that Fsh cells become differentiated before hatching and that sexual dimorphism in Fsh cells occurs transiently after sex determination in medaka.
巻・号 204
ページ 173-80
公開日 2014-8-1
DOI 10.1016/j.ygcen.2014.04.036
PII S0016-6480(14)00186-5
PMID 24859259
MeSH Animals Female Follicle Stimulating Hormone, beta Subunit / metabolism* Glycoprotein Hormones, alpha Subunit / metabolism* Gonads / embryology* Immunoenzyme Techniques In Situ Hybridization Luteinizing Hormone, beta Subunit / metabolism* Male Oryzias Pituitary Gland, Anterior / metabolism* Sex Differentiation* / physiology Sexual Maturation
IF 2.428
引用数 7
WOS 分野 ENDOCRINOLOGY & METABOLISM
リソース情報
メダカ d-rR/TOKYO (MT837)