論文 - 詳細
| RRC ID | 36339 |
|---|---|
| 著者 | Yasukochi Y, Ohno M, Shibata F, Jouraku A, Nakano R, Ishikawa Y, Sahara K. |
| タイトル | A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm. |
| ジャーナル | Heredity (Edinb) |
| Abstract |
A significant feature of the genomes of Lepidoptera, butterflies and moths, is the high conservation of chromosome organization. Recent remarkable progress in genome sequencing of Lepidoptera has revealed that syntenic gene order is extensively conserved across phylogenetically distant species. The ancestral karyotype of Lepidoptera is thought to be n=31; however, that of the most well-studied moth, Bombyx mori, is n=28, and diverse studies suggest that three chromosomal fusion events occurred in this lineage. To identify the boundaries between predicted ancient fusions involving B. mori chromosomes 11, 23 and 24, we constructed fluorescence in situ hybridization (FISH)-based chromosome maps of the European corn borer, Ostrinia nubilalis (n=31). We first determined a 511 Mb genomic sequence of the Asian corn borer, O. furnacalis, a congener of O. nubilalis, and isolated bacterial artificial chromosomes and fosmid clones that were expected to localize in candidate regions for the boundaries using these sequences. Combined with FISH and genetic analysis, we narrowed down the candidate regions to 40 kb-1.5 Mb, in strong agreement with a previous estimate based on the genome of a butterfly, Melitaea cinxia. The significant difference in the lengths of the candidate regions where no functional genes were observed may reflect the evolutionary time after fusion events. |
| 巻・号 | 116(1) |
| ページ | 75-83 |
| 公開日 | 2016-1-1 |
| DOI | 10.1038/hdy.2015.72 |
| PII | hdy201572 |
| PMID | 26264548 |
| PMC | PMC4675876 |
| MeSH | Animals Biological Evolution* Chromosome Mapping* Chromosomes, Artificial, Bacterial Genes, Insect Genome, Insect* Genotype In Situ Hybridization, Fluorescence Male Moths / genetics* Synteny* Telomere / genetics Zea mays |
| IF | 3.436 |
| 引用数 | 13 |
| WOS 分野 | GENETICS & HEREDITY ECOLOGY EVOLUTIONARY BIOLOGY |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| カイコ | p50 |